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Product Info Summary
| SKU: | PA1788 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HLA DMB/HLA-DMB Antibody Picoband®
SKU/Catalog Number
PA1788
BA3756 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HLA DMB/HLA-DMB Antibody catalog # PA1788. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA DMB/HLA-DMB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1788)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HLA DMB.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1788 is reactive to HLA-DMB in Human
Observed Molecular Weight
29 kDa
Calculated molecular weight
28.9 kDa
Background of HLA-DMB
HLA-DMB (major histocompatibility complex, class II, DM beta), also known as D6S221E, RING7, HLA-DM histocompatibility type, beta chain, HLADMB or RING7, is a protein that in humans is encoded by the HLA-DMB gene. The HLA-DMB gene is mapped on 6p21.32. HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. HLA-DMA and -DMB appear to encode subunits of a functional heterodimer that is critical in the pathway of class II antigen presentation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1788 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: JURKAT Whole Cell, JURKAT Whole Cell, RAJI Whole Cell, HUT Whole Cell
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-HLA DMB antibody, PA1788, Western blotting
All lanes: Anti HLA DMB (PA1788) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
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Anti-HLA DMB antibody, PA1788, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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miR-411-5p activate p38MAPK pathway by targeting SPRY4-induced terminal differentiation of RMS. ( a ) Western blot analysis of p38MAPK activation (P-p38MAPK: p-38) in RMS cells treated with HA-tagged MKK6EE protein-expressing pcDNA3, SPRY4 siRNA, or both. ( b ) Western blot analysis of p38MAPK activation (P-p38MAPK:p-38) in RMS cells treated with miR-411-5p-M, miR-411-5p-I, and MKK6EE protein-expressing pcDNA3 plus miR-411-5p-M. ( c ) Terminal differentiation of RD cells after treatment with SPRY4 siRNA or miR-411-5p-M for 6 and 48 h. Cells stained with apoptosis marker (caspase-3); cell cycle was analyzed using propidium iodide (PI) and showed changed morphology (MHC and myosin) after activation of p38MAPK caused by SPRY4 siRNA or miR-411-5p-M. Scale bars: panel (Caspase-3)=100 μ m, panel (MHC and Myosin)=20 μ m. ( d ) Quantitation of terminal differentiation of RD cells after treatment with SPRY4 siRNA or miR-411-5p-M for 6 and 48 h. ( e ) Graphical representation of cell cycle phase proportions in RD cells after treatment with miR-411-5p-M for 6 and 48 h. ( f ) Proliferation of RD cells was evaluated by [ 3 H] thymidine incorporation assay after treatment with SPRY4 siRNA or miR-411-5p-M for 4 days. The amount of [ 3 H] thymidine incorporated was measured with a liquid scintillation counter. The OD at 557 nm was determined using a microplate reader. ( g ) Western blot analysis of cleaved caspase-3 in RMS cells treated with SPRY4 siRNA or miR-411-5p-M for 24 and 72 h. Each assay ( a – g ) was conducted at least three times independently. Error bars indicate S.D. * P <0.05; ** P <0.01; *** P <0.005
Index in PubMed under a CC BY license. PMID: 26291313
Specific Publications For Anti-HLA DMB/HLA-DMB Antibody Picoband® (PA1788)
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4 Customer Q&As for Anti-HLA DMB/HLA-DMB Antibody Picoband®
Question
We are currently using anti-HLA DMB/HLA-DMB antibody PA1788 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The anti-HLA DMB/HLA-DMB antibody (PA1788) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17
Question
Will PA1788 anti-HLA DMB/HLA-DMB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
You can see on the product datasheet, PA1788 anti-HLA DMB/HLA-DMB antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-20
Question
Would anti-HLA DMB/HLA-DMB antibody PA1788 work for IHC with plasma?
J. Banerjee
Verified customer
Asked: 2015-07-08
Answer
According to the expression profile of plasma, HLA-DMB is highly expressed in plasma. So, it is likely that anti-HLA DMB/HLA-DMB antibody PA1788 will work for IHC with plasma.
Boster Scientific Support
Answered: 2015-07-08
Question
I have a question about product PA1788, anti-HLA DMB/HLA-DMB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Yang
Verified customer
Asked: 2014-10-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1788 anti-HLA DMB/HLA-DMB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-10-02
