Anti-HLA-G Purified Azide Free Monoclonal Antibody

Product Name

Anti-HLA-G Purified Azide Free Monoclonal Antibody

View all HLA-G Antibodies

SKU/Catalog Number

M01235-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-HLA-G Purified Azide Free Monoclonal Antibody (Catalog# M01235-2). Tested in Flow Cytometry, IP, IHC-F, ICC, ELISA application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-HLA-G Purified Azide Free Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01235-2)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, azide-free

Clonality

Monoclonal

Clone Number

MEM-G/9

Isotype

Mouse IgG1

Immunogen

Recombinant human HLA-G refolded with beta2-microglobulin and peptide. The antibody MEM-G/9 reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).

Reactive Species

M01235-2 is reactive to HLA-G in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

38.2 kDa

Background of HLA-G

Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01235-2 is guaranteed for ELISA, Flow Cytometry, IP, IHC-F, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 0.3-4 μg/ml; positive control: JEG-3 human choriocarcinoma cell line.
Immunocytochemistry: 2-5 μg/ml.
ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 μg/ml, detection antibody (biotin or peroxidase conjugate) 1 μg/ml.
Immunohistochemistry: 1-4 μg/ml.

Validation Images & Assay Conditions

See full target information HLA-G

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