Anti-VISTA VSIR Monoclonal Antibody [4C4]

Product Name

Anti-VISTA VSIR Monoclonal Antibody [4C4]

View all VSIR Antibodies

SKU/Catalog Number

M32320

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-VISTA VSIR Monoclonal Antibody [4C4] (Catalog # M32320). Tested in ELISA, WB, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

VISTA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-VISTA VSIR Monoclonal Antibody [4C4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32320)

Host

Mouse

Contents

VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

Clone: 4C4

Isotype

IgG1

Immunogen

VISTA antibody was raised against the extracellular domain of human VISTA.

Reactive Species

M32320 is reactive to VSIR in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

33.9 kDa

Background of VSIR

VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M32320 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

VISTA antibody can be used for detection of VISTA by Western blot at 0.25 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information VSIR

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