This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CD274 antibody (M00109-3) performs well in ELISA, IF, IHC-P, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Immunofluorescence Validation of PD-L1 in Transfected 293 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed PD-L1 transfected 293 cells labeling PD-L1 with M00109-3 at 2 μg/mL, followed by goat anti-mouse IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed PD-L1 transfected 293 cells labeling PD-L1 with M00109-3 at 2 μg/mL, followed by goat anti-mouse IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Bundle Save
Anti-PDL1 CD274 Monoclonal Antibody [6H10]
CD274 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Mouse | ELISA, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 0.25 - 4 μg/mL; IF: 2 μg/mL; IHC-P: 5 μg/mL; ICC: 1 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry
Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-PDL1 CD274 Monoclonal Antibody [6H10]
View all CD274 Antibodies
View all CD274 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Mouse
Contents
PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 6H10
Isotype
IgG1
Immunogen
Anti-PD-L1 antibody was raised against the extracellular domain of human PD-L1.
Cross-Reactivity
PD-L1 Antibody has no cross-reactivity to PD-L2.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
33.3 kDa
Background of CD274
PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3). Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4).
Cite This Product
Anti-PDL1 CD274 Monoclonal Antibody [6H10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00109-3)
Review
Have you used Anti-PDL1 CD274 Monoclonal Antibody [6H10]?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-PDL1 CD274 Monoclonal Antibody [6H10] performs excellently in relevant applications for ELISA, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00109-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026