Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3)

Product Name

Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3)

View all HLA-DQA1 Antibodies

SKU/Catalog Number

M00232

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3) (Catalog# M00232). Tested in FACS application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00232)

Host

Mouse

Contents

100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

SPVL3

Isotype

IgG2a Kappa

Immunogen

Cells of T4 and CTL clone HG-38

Reactive Species

M00232 is reactive to HLA-DQA1 in Human

Calculated molecular weight

27.8 kDa

Background of HLA-DQA1

Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00232 is guaranteed for FACS Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FACS: 0.5-1 µg/10^6 cells

Validation Images & Assay Conditions

See full target information HLA-DQA1

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis