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- Table of Contents
Il1b antibody (PA1351) performs well in WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Anti-IL-1 beta antibody, PA1351, Western blotting
Lane 1: Recombinant Mouse IL-1 beta Protein 10ng
Lane 2: Recombinant Mouse IL-1 beta Protein 5ng
Lane 3: Recombinant Mouse IL-1 beta Protein 2.5ng
Lane 1: Recombinant Mouse IL-1 beta Protein 10ng
Lane 2: Recombinant Mouse IL-1 beta Protein 5ng
Lane 3: Recombinant Mouse IL-1 beta Protein 2.5ng
Designing a Western blot for IL1B? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the IL1B Western Blot GuideBundle Save
Anti-IL1 beta/IL1B Antibody Picoband®
Il1b antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | WB |
Tested Applications
Il1b antibody was positively detected by WB in:
recombinant mouse IL-1 beta protein
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IL1 beta/IL1B Antibody Picoband®
View all IL1B Antibodies
View all IL1B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL-1 beta, different from the related rat sequence by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
20 kDa
Calculated Molecular Weight
30.9 kDa
Background of Il1b
Interleukin-1 beta (IL-1beta) also know as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene, which localizes to the long arm of chromosome at position 2q13-2q21 between two fragile sites. Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses.2 The human IL-1 family plays an important role in the pathogenesis of many diseases and functions as a key mediator of the host response to various infectious, inflammatory, and immunologic challenges.
Cite This Product
Anti-IL1 beta/IL1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1351)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rabbit
88%
Moderate Homology
Dog
81%
Moderate Homology
Rat
75%
Moderate Homology
Human
69%
Low homology
Cow
69%
Low homology
Sheep
69%
Low homology
Monkey
69%
Low homology
Zebrafish
19%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Il1b antibody
Publications
Application:
Species:
Carnosic acid alleviates brain injury through NF‑κB‑regulated inflammation and Caspase‑3‑associated apoptosis in high fat‑induced mouse models
Published: 2019-05-27
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.85
Species: Mouse
PMID: 31180544
Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo
Published: 2018-01-11
Journal: MOLECULAR IMMUNOLOGY
Application: WB
Impact Factor: 3.19
Species: Mouse
PMID: 29331803
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Product Promise
We promise Anti-IL1 beta/IL1B Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PA1351
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 16, 2026