|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-IL-1 beta Antibody|
|Description||Rabbit IgG polyclonal antibody for Interleukin-1 beta(IL1B) detection. Tested with WB in Mouse;Rat.|
|Cite This Product||Anti-IL-1 beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1351)|
|Replacement Item||This antibody may replace the following items: sc-1250|sc-12742|sc-130323|sc-23459|sc-32294|sc-52012|sc-52013|sc-52771|sc-7884 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL-1 beta(250-265aa FLGNNSGQDIIDFTME), different from the related rat sequence by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Interleukin-1 beta(IL-1 beta)|
|Molecular Weight||30931 MW|
|Protein Function||Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.|
|Subcellular Localization||Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.|
|Alternative Names||Interleukin-1 beta;IL-1 beta;Il1b;|
Background for Interleukin-1 beta(IL-1 beta)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-IL-1 beta Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-IL-1 beta Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Mouse IL-1 beta Protein 10ng
Lane 2: Recombinant Mouse IL-1 beta Protein 5ng
Lane 3: Recombinant Mouse IL-1 beta Protein 2.5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,