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Product Info Summary
| SKU: | PA1351 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL1 beta/IL1B Antibody Picoband®
SKU/Catalog Number
PA1351
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL1 beta/IL1B Antibody catalog # PA1351. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL1 beta/IL1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1351)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL-1 beta, different from the related rat sequence by four amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1351 is reactive to Il1b in Mouse, Rat
Observed Molecular Weight
20 kDa
Calculated molecular weight
30.9 kDa
Background of Il1b
Interleukin-1 beta (IL-1beta) also know as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene, which localizes to the long arm of chromosome at position 2q13-2q21 between two fragile sites. Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses.2 The human IL-1 family plays an important role in the pathogenesis of many diseases and functions as a key mediator of the host response to various infectious, inflammatory, and immunologic challenges.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1351 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
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Anti-IL-1 beta antibody, PA1351, Western blotting
Lane 1: Recombinant Mouse IL-1 beta Protein 10ng
Lane 2: Recombinant Mouse IL-1 beta Protein 5ng
Lane 3: Recombinant Mouse IL-1 beta Protein 2.5ng
Specific Publications For Anti-IL1 beta/IL1B Antibody Picoband® (PA1351)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-IL1 beta/IL1B Antibody Picoband®
Question
Is there a BSA free version of anti-IL1 beta/IL1B antibody PA1351 available?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL1 beta/IL1B antibody PA1351 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-25
Question
We are currently using anti-IL1 beta/IL1B antibody PA1351 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
The anti-IL1 beta/IL1B antibody (PA1351) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for monocyte using anti-IL1 beta/IL1B antibody PA1351. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
I was wanting to use your anti-IL1 beta/IL1B antibody for WB for mouse monocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse monocyte identification?
B. Thomas
Verified customer
Asked: 2019-12-23
Answer
As indicated on the product datasheet, PA1351 anti-IL1 beta/IL1B antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse monocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-23
Question
Is a blocking peptide available for product anti-IL1 beta/IL1B antibody (PA1351)?
V. Krishna
Verified customer
Asked: 2019-09-19
Answer
We do provide the blocking peptide for product anti-IL1 beta/IL1B antibody (PA1351). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-19
Question
We need to test anti-IL1 beta/IL1B antibody PA1351 on mouse monocyte for research purposes, then I may be interested in using anti-IL1 beta/IL1B antibody PA1351 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The products we sell, including anti-IL1 beta/IL1B antibody PA1351, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-01
Question
Is this PA1351 anti-IL1 beta/IL1B antibody reactive to the isotypes of IL1B?
R. Edwards
Verified customer
Asked: 2019-03-06
Answer
The immunogen of PA1351 anti-IL1 beta/IL1B antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL-1 beta(250-265aa FLGNNSGQDIIDFTME), different from the related rat sequence by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-06
Question
See below the WB image, lot number and protocol we used for monocyte using anti-IL1 beta/IL1B antibody PA1351. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-28
Question
Would PA1351 anti-IL1 beta/IL1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
As indicated on the product datasheet, PA1351 anti-IL1 beta/IL1B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-01
Question
Does anti-IL1 beta/IL1B antibody PA1351 work for WB with monocyte?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
According to the expression profile of monocyte, IL1B is highly expressed in monocyte. So, it is likely that anti-IL1 beta/IL1B antibody PA1351 will work for WB with monocyte.
Boster Scientific Support
Answered: 2018-07-12
Question
We have seen staining in rat histiocytic lymphoma. Do you have any suggestions? Is anti-IL1 beta/IL1B antibody supposed to stain histiocytic lymphoma positively?
J. Mangal
Verified customer
Asked: 2018-06-27
Answer
From literature histiocytic lymphoma does express IL1B. From Uniprot.org, IL1B is expressed in smooth muscle tissue, leukocyte, histiocytic lymphoma, monocyte, lung, skin, macrophage, among other tissues. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2018-06-27
Question
Does anti-IL1 beta/IL1B antibody PA1351 work on canine WB with leukocyte?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Our lab technicians have not validated anti-IL1 beta/IL1B antibody PA1351 on canine. You can run a BLAST between canine and the immunogen sequence of anti-IL1 beta/IL1B antibody PA1351 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine leukocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-18
Question
We were well pleased with the WB result of your anti-IL1 beta/IL1B antibody. However we have been able to see positive staining in lung cytoplasm using this antibody. Is that expected? Could you tell me where is IL1B supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
From literature, lung does express IL1B. Generally IL1B expresses in cytoplasm, cytosol. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2017-06-21
Question
I see that the anti-IL1 beta/IL1B antibody PA1351 works with WB, what is the protocol used to produce the result images on the product page?
P. Johnson
Verified customer
Asked: 2016-09-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-09-06
Question
My question regarding product PA1351, anti-IL1 beta/IL1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Li
Verified customer
Asked: 2015-06-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1351 anti-IL1 beta/IL1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-22
