Human IL-1 beta ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human IL-1 beta ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.15 pg/ml. Cited in 8 publication(s).

Product Info Summary

SKU: EZ0392
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human IL-1 beta ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all IL-1 beta/IL-1F2 products

SKU/Catalog Number

EZ0392

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human IL-1 beta ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.15 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-1 beta ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0392)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A117-S269

Sensitivity

<0.15 pg/ml

Assay Range

3.9 pg/ml - 250 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0392 is reactive to IL1B in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Application Guarantee

EZ0392 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0392-CA Rabbit anti- human IL1B polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0392-DA Biotinylated goat anti- human IL1B polyclonal antibody (Detection Antibody) 500 µl, 25 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0392-ST Lyophilized recombinant human IL1B standard 1 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03.97.815.631.262.5125250
O.D.0.0220.0740.1050.2490.4230.7071.0951.830

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)23561582662173
Standard deviation13.48.51.63.912.4
CV (%)4.5%6.1%5.4%6.3%6.4%7.2%

Gene/Protein Information For IL1B (Source: Uniprot.Org, NCBI)

Uniprot ID

P01584

Gene ID

3553

Gene Name

IL1B

Full Name

Interleukin-1 beta

Weight

30.748kDa

Superfamily

IL-1 family

Alternative Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL1B, check out the IL1B Infographic

IL1B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EZ0392 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-%u03BAB and IL-1%u03B2 %u2026

Protection of SAL B with H9C2 cells

Increased Age-Related Cardiac Dysfunction in Bradykinin B2 Receptor%u2013Deficient Mice

HoxA13 Stimulates Myometrial Cells to Secrete IL-1%u03B2 and Enhance the Expression of Contraction-Associated Proteins

Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosus

Liu X, Zhang X, Ding Y, Zhou W, Tao L, Lu P, Wang Y, Hu R. Antioxid Redox Signal. 2017 Jan 1;26(1):28-43. doi: 10.1089/ars.2015.6615. Epub 2016 Aug 9. Nuclear Factor E2-Related Factor-2 Negatively Regulates NLRP3 Inflammasome Activity by Inhibitin...

Zhou W, Liu X, Zhang X, Tang J, Li Z, Wang Q, Hu R. Oncotarget. 2017 Jul 22;8(35):58903-58917. doi: 10.18632/oncotarget.19440. eCollection 2017 Aug 29. Oroxylin A inhibits colitis by inactivating NLRP3 inflammasome

Sun, G., Yang, W., Zhang, Y., & Zhao, M. (2017). Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Experimental Animals. Advance online publication. doi: 10.1538/expanim.16-0102

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