Anti-hnRNP D0 Antibody

AUF1 antibody

Boster Bio Anti-hnRNP D0 Antibody catalog # A02895-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A02895-2
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-hnRNP D0 Antibody

View all AUF1 Antibodies

SKU/Catalog Number

A02895-2

Size

100μl

Form

Liquid

Description

Boster Bio Anti-hnRNP D0 Antibody catalog # A02895-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-hnRNP D0 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02895-2)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A6

Isotype

IgG

Immunogen

Synthesized peptide derived from human hnRNP D0 around the non-phosphorylation site of S83.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02895-2 is reactive to HNRNPD in Human, Mouse, Rat

Applications

A02895-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

38.434kDa

Background of AUF1

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of a-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Emamian ES, et al. (2004) J Neurosci. 24(7): 1561-4
Palmer, C.L. et al. (2005) Pharmacol. Rev. 57, 253-277.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For HNRNPD (Source: Uniprot.org, NCBI)

Gene Name

HNRNPD

Full Name

Heterogeneous nuclear ribonucleoprotein D0

Weight

38.434kDa

Alternative Names

AUF1A; heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein1, 37kDa); heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA-binding protein1, 37kD); heterogeneous nuclear ribonucleoprotein D0; hnRNPD0; type A HNRNPD AUF1, AUF1A, HNRPD, P37, hnRNPD0 heterogeneous nuclear ribonucleoprotein D heterogeneous nuclear ribonucleoprotein D0|ARE-binding protein AUFI, type A|AU-rich element RNA binding protein 1, 37kDa|hnRNP D0

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HNRNPD, check out the HNRNPD Infographic

HNRNPD infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HNRNPD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-hnRNP D0 Antibody

Question

Does anti-hnRNP D0 antibody A02895-2 work on monkey WB with blood?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

Our lab technicians have not tested anti-hnRNP D0 antibody A02895-2 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-hnRNP D0 antibody A02895-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-09

Question

Is this A02895-2 anti-hnRNP D0 antibody reactive to the isotypes of HNRNPD?

Verified Customer

Verified customer

Asked: 2019-02-19

Answer

The immunogen of A02895-2 anti-hnRNP D0 antibody is Synthesized peptide derived from human hnRNP D0 around the non-phosphorylation site of S83. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-19

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-hnRNP D0 antibody A02895-2. Please let me know if you require anything else.

E. Bhatt

Verified customer

Asked: 2018-01-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-17

Question

I was wanting to use your anti-hnRNP D0 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

M. Jackson

Verified customer

Asked: 2014-09-02

Answer

You can see on the product datasheet, A02895-2 anti-hnRNP D0 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-09-02

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-hnRNP D0 antibody A02895-2. Let me know if you need anything else.

B. Jones

Verified customer

Asked: 2014-06-16

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-06-16

Order DetailsPrice
A02895-2

100uL

$399

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