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Product Info Summary
| SKU: | A02895-2 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-hnRNP D0 Antibody
SKU/Catalog Number
A02895-2
Size
100μl
Form
Liquid
Description
Boster Bio Anti-hnRNP D0 Antibody catalog # A02895-2. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-hnRNP D0 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02895-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human hnRPD. AA range:49-98
Reactive Species
A02895-2 is reactive to HNRNPD in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02895-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using hnRPD Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from 293, Jurkat, 3T3, and COLO205 cells, using hnRPD Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using hnRPD Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-hnRNP D0 Antibody (A02895-2)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-hnRNP D0 Antibody
Question
Does anti-hnRNP D0 antibody A02895-2 work on monkey WB with blood?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
Our lab technicians have not tested anti-hnRNP D0 antibody A02895-2 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-hnRNP D0 antibody A02895-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-09
Question
Is this A02895-2 anti-hnRNP D0 antibody reactive to the isotypes of HNRNPD?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
The immunogen of A02895-2 anti-hnRNP D0 antibody is Synthesized peptide derived from human hnRNP D0 around the non-phosphorylation site of S83. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-19
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-hnRNP D0 antibody A02895-2. Please let me know if you require anything else.
E. Bhatt
Verified customer
Asked: 2018-01-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-17
Question
I was wanting to use your anti-hnRNP D0 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
M. Jackson
Verified customer
Asked: 2014-09-02
Answer
You can see on the product datasheet, A02895-2 anti-hnRNP D0 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-09-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-hnRNP D0 antibody A02895-2. Let me know if you need anything else.
B. Jones
Verified customer
Asked: 2014-06-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-16
