Anti-Homeobox protein DBX1 DBX1 Antibody

Product Name

Anti-Homeobox protein DBX1 DBX1 Antibody

View all DBX1 Antibodies

SKU/Catalog Number

A14046

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Homeobox protein DBX1 DBX1 Antibody (Catalog # A14046). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

DBX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Homeobox protein DBX1 DBX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14046)

Host

Rabbit

Contents

DBX1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DBX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human DBX1. The immunogen is located within amino acids 100 - 150 of DBX1.

Cross-reactivity

BX1 antibody is predicted to not cross-react with other DBX protein family members

Reactive Species

A14046 is reactive to DBX1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

37.3 kDa

Background of DBX1

DBX1 is implicated in patterning the central nervous system during embryogenesis. DBX1 homeodomain transcription factor is expressed in progenitors which are critical in establishing cell fate allocation and cell diversity. DBX1 has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneurons, V0 and V1 neurons. DBX1-derived neurons are essential for the transgenic expression and generation of respiratory behavior both in vitro and in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14046 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DBX1 antibody can be used for detection of DBX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information DBX1

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