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- Table of Contents
NANOG antibody (A00153) performs well in ELISA, IF, IHC-P, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunoflouorescence Validation of NANOG in HeLa Cells
Immunofluorescent analysis of PFA-fixed HeLa cells labeling NANOG with A00153 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Immunofluorescent analysis of PFA-fixed HeLa cells labeling NANOG with A00153 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
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Anti-Homeobox protein NANOG NANOG Antibody
NANOG antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-2 μg/mL; ICC/IF: 20 μg/mL; IHC: 1-5 μg/mL.
Antibody validated: Western Blot in human
mouse and rat samples; Immunohistochemistry in human
mouse and rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Homeobox protein NANOG NANOG Antibody
View all NANOG Antibodies
View all NANOG Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
NANOG antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
NANOG Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG. The immunogen is located within amino acids 130 - 180 of NANOG.
Observed Molecular Weight
35 kDa
Calculated Molecular Weight
34.6 kDa
Background of NANOG
Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Cite This Product
Anti-Homeobox protein NANOG NANOG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00153)
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Product Promise
We promise Anti-Homeobox protein NANOG NANOG Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00153
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026