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Product Info Summary
| SKU: | A00153 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-Homeobox protein NANOG NANOG Antibody
SKU/Catalog Number
A00153
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Homeobox protein NANOG NANOG Antibody (Catalog # A00153). Tested in ELISA, WB, IHC-P, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NANOG antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Homeobox protein NANOG NANOG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00153)
Host
Rabbit
Contents
NANOG Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG. The immunogen is located within amino acids 130 - 180 of NANOG.
Reactive Species
A00153 is reactive to NANOG in Human, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
34.6 kDa
Background of NANOG
Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00153 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; ICC/IF: 20 μg/mL; IHC: 1-5 μg/mL.
Validation Images & Assay Conditions
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Immunoflouorescence Validation of NANOG in HeLa Cells
Immunofluorescent analysis of PFA-fixed HeLa cells labeling NANOG with A00153 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
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WB Validation in HeLa Cells
Loading: 15 μg of lysate.
Antibodies: NANOG A00153, 2 μg/mL , 1 h incubation at RT in 8% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
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Western Blot Validation in Mouse and Rat Testis
Loading: 15 μg of lysates per lane.
Antibodies: NANOG A00153, 2 μg/mL, 1h incubation at RT in 8% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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Immunohistochemistry Validation of NANOG in Human Testis
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-NANOG antibody (A00153) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of NANOG in Mouse Testis
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NANOG antibody (A00153) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of NANOG in Rat Testis
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NANOG antibody (A00153) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-Homeobox protein NANOG NANOG Antibody (A00153)
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