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Product Info Summary
|Application:||IHC, ICC, WB|
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Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100)
Boster Bio Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) catalog # MA1075. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1075)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Recombinant human p19INK4d.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
MA1075 is reactive to Cdkn2d in Human
MA1075 is guaranteed for IHC, ICC, WB Boster Guarantee
Background of p19 INK4d
Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin-dependent kinases. P19 (INK4d) also kowns as cyclin-dependent kinase inhibitor 2D, is one of the novel members of the mouse INK4 gene family. Okuda et al. (1995) described the cloning of the human INK4d gene (CDKN2D). The predicted 166-amino acid protein is 86% identical to the mouse protein and about 45% identical to other human INK4 family members.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of p19INK4d using anti-p19INK4d antibody (MA1075).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p19INK4d antigen affinity purified monoclonal antibody (Catalog # MA1075) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p19INK4d at approximately 18 kDa. The expected band size for p19INK4d is at 18 kDa.
Gene/Protein Information For Cdkn2d (Source: Uniprot.org, NCBI)
Cyclin-dependent kinase 4 inhibitor D
CDKN2 cyclin-dependent kinase inhibitor family
CDK inhibitor p19INK4d; cell cycle inhibitor, Nur77 associating protein; cyclin-dependent kinase 4 inhibitor D p19; cyclin-dependent kinase 4 inhibitor D; cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4); inhibitor of cyclin-dependent kinase 4d; INK4D; p19; p19-INK4D Cdkn2d|INK, INK4d, p1, p19, p19IN, p19INK4d|cyclin dependent kinase inhibitor 2D|cyclin-dependent kinase 4 inhibitor D|cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)|p19-INK4d*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Cdkn2d, check out the Cdkn2d Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cdkn2d: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
No publications found for MA1075
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