Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100)

Boster Bio Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) catalog # MA1075. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: MA1075
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC, ICC, WB

Product Name

Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100)

See all p19 INK4d products

SKU/Catalog Number

MA1075

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) catalog # MA1075. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1075)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DCS-100

Isotype

Mouse IgG1

Immunogen

Recombinant human p19INK4d.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1075 is reactive to Cdkn2d in Human

Applications

MA1075 is guaranteed for IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For Cdkn2d (Source: Uniprot.Org, NCBI)

Uniprot ID

Q60773

Gene ID

12581

Gene Name

Cdkn2d

Full Name

Cyclin-dependent kinase 4 inhibitor D

Weight

17.809kDa

Superfamily

CDKN2 cyclin-dependent kinase inhibitor family

Alternative Names

CDK inhibitor p19INK4d; cell cycle inhibitor, Nur77 associating protein; cyclin-dependent kinase 4 inhibitor D p19; cyclin-dependent kinase 4 inhibitor D; cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4); inhibitor of cyclin-dependent kinase 4d; INK4D; p19; p19-INK4D Cdkn2d|INK, INK4d, p1, p19, p19IN, p19INK4d|cyclin dependent kinase inhibitor 2D|cyclin-dependent kinase 4 inhibitor D|cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)|p19-INK4d

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cdkn2d, check out the Cdkn2d Infographic

Cdkn2d infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cdkn2d: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100)

Question

We have seen staining in human bone marrow. What should we do? Is anti-p19INK4d antibody (Monoclonal, DCS-100) supposed to stain bone marrow positively?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

From what I have seen in literature bone marrow does express CDKN2D. From what I have seen in Uniprot.org, CDKN2D is expressed in blood, bone marrow, thymus, muscle, platelet, among other tissues. Regarding which tissues have CDKN2D expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 8575754
Muscle, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Thymus, Pubmed ID: 7739548

Boster Scientific Support

Answered: 2020-04-17

Question

My colleagues were happy with the WB result of your anti-p19INK4d antibody (Monoclonal, DCS-100). However we have seen positive staining in bone marrow nucleus. using this antibody. Is that expected? Could you tell me where is CDKN2D supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

According to literature, bone marrow does express CDKN2D. Generally CDKN2D expresses in nucleus. Regarding which tissues have CDKN2D expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 8575754
Muscle, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Thymus, Pubmed ID: 7739548

Boster Scientific Support

Answered: 2020-01-30

Question

We are currently using anti-p19INK4d antibody (Monoclonal, DCS-100) MA1075 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-01-24

Answer

The anti-p19INK4d antibody (Monoclonal, DCS-100) (MA1075) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-01-24

Question

We purchased anti-p19INK4d antibody (Monoclonal, DCS-100) for IHC on blood in the past. I am using human, and We want to use the antibody for WB next. My question regards examining blood as well as platelet in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-04-24

Answer

I have checked the website and datasheets of our anti-p19INK4d antibody (Monoclonal, DCS-100) and I see that MA1075 has been tested on human in both IHC and WB. Thus MA1075 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-04-24

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