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Product Info Summary
| SKU: | MA1075 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100)
SKU/Catalog Number
MA1075
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) catalog # MA1075. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1075)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
DCS-100
Isotype
Mouse IgG1
Immunogen
Recombinant human p19INK4d.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1075 is reactive to Cdkn2d in Human
Observed Molecular Weight
18 kDa
Calculated molecular weight
17.8 kDa
Background of Cdkn2d
Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin-dependent kinases. P19 (INK4d) also kowns as cyclin-dependent kinase inhibitor 2D, is one of the novel members of the mouse INK4 gene family. Okuda et al. (1995) described the cloning of the human INK4d gene (CDKN2D). The predicted 166-amino acid protein is 86% identical to the mouse protein and about 45% identical to other human INK4 family members.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1075 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Positive Control
WB: human MOLT-4 whole cell, human HEL whole cell, human Jurkat whole cell
Validation Images & Assay Conditions
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Western blot analysis of p19INK4d using anti-p19INK4d antibody (MA1075).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p19INK4d antigen affinity purified monoclonal antibody (Catalog # MA1075) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p19INK4d at approximately 18 kDa. The expected band size for p19INK4d is at 18 kDa.
Specific Publications For Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) (MA1075)
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Customer Q&As
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4 Customer Q&As for Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100)
Question
We have seen staining in human bone marrow. What should we do? Is anti-p19INK4d antibody (Monoclonal, DCS-100) supposed to stain bone marrow positively?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
From what I have seen in literature bone marrow does express CDKN2D. From what I have seen in Uniprot.org, CDKN2D is expressed in blood, bone marrow, thymus, muscle, platelet, among other tissues. Regarding which tissues have CDKN2D expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 8575754
Muscle, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Thymus, Pubmed ID: 7739548
Boster Scientific Support
Answered: 2020-04-17
Question
My colleagues were happy with the WB result of your anti-p19INK4d antibody (Monoclonal, DCS-100). However we have seen positive staining in bone marrow nucleus. using this antibody. Is that expected? Could you tell me where is CDKN2D supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
According to literature, bone marrow does express CDKN2D. Generally CDKN2D expresses in nucleus. Regarding which tissues have CDKN2D expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 8575754
Muscle, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Thymus, Pubmed ID: 7739548
Boster Scientific Support
Answered: 2020-01-30
Question
We are currently using anti-p19INK4d antibody (Monoclonal, DCS-100) MA1075 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
The anti-p19INK4d antibody (Monoclonal, DCS-100) (MA1075) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-24
Question
We purchased anti-p19INK4d antibody (Monoclonal, DCS-100) for IHC on blood in the past. I am using human, and We want to use the antibody for WB next. My question regards examining blood as well as platelet in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
I have checked the website and datasheets of our anti-p19INK4d antibody (Monoclonal, DCS-100) and I see that MA1075 has been tested on human in both IHC and WB. Thus MA1075 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-24
