Anti-HOXA4 Antibody

Rabbit IgG polyclonal antibody for Homeobox protein Hox-A4(HOXA4) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

SKU PA1603
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, ICC, WB

Overview

Product Name Anti-HOXA4 Antibody
See all HOXA4 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1603
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Polyclonal antibody for HOXA4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. HOXA4 information: Molecular Weight: 34499 MW; Subcellular Localization: Nucleus; Tissue Specificity: Embryonic nervous system.
Cite This Product Anti-HOXA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1603)
Specificity Anti-HOXA4 Antibody (PA1603) reacts with Human, Mouse, Rat HOXA4, in native form and recombinant. Superfamily members of HOXA4 are not reactive to PA1603.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-HOXA4 Antibody (PA1603) was raised against A synthetic peptide corresponding to a sequence at the N-terminus of human HOXA4(9-26aa NSNYIEPKFPPFEEYAQH), different from the related mouse sequence by two amino acids..
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-HOXA4 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-HOXA4 Antibody (PA1603).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Images And Assay Conditions

/antibody/pa1603 1 WB anti hoxa4 antibody.jpg

Anti-HOXA4 antibody, PA1603, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: PC-12 Cell Nuclear Lysate

/antibody/pa1603 2 IHC anti hoxa4 antibody.jpg

Anti-HOXA4 antibody, PA1603, IHC(P)
IHC(P): Human Mammary Cancer Tissue

/antibody/pa1603 3 IF anti hoxa4 antibody.jpg

Anti-HOXA4 antibody, PA1603, ICC
ICC: HELA Cell

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q00056
Gene Name HOXA4
Protein Name Homeobox protein Hox-A4
Tissue Specificity Embryonic nervous system.
Alternative Names Homeobox protein Hox-A4;Homeobox protein Hox-1.4;Homeobox protein Hox-1D;HOXA4;HOX1D;Show more
Subcellular Localization Nucleus.
Molecular Weight 34499 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT- 3'.
Research Areas Developmental Families, Domain Families, Epigenetics And Nuclear Signaling, Transcription, Transcription Factors

*You can search these to find other products in these research areas.
Background HOXA4(HOMEOBOX A4), also known as HOX1D or HOMOLOG OF, is a protein that in humans is encoded by the HOXA4 gene, which is also part of the A cluster on chromosome 7. HOXA4 encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Its cytogenetic location is 7q15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-HOXA4 Antibody (PA1603)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1603)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships next business day.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Publications

PBX3 and MEIS1 Cooperate in Hematopoietic Cells to Drive Acute Myeloid Leukemias Characterized by a Core Transcriptome of the MLL-Rearranged Disease

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.