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Pack Size:100μg/vial
Validated Species:Human
Application:IHC, ICC, WB
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Product Name Anti-CYP2U1 Antibody
SKU/Catalog Number PA2022
Description Rabbit IgG polyclonal antibody for Cytochrome P450 2U1(CYP2U1) detection. Tested with WB, IHC-P, ICC in Human.
Cite This Product Anti-CYP2U1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2022)
Replacement Item This antibody may replace the following items: sc-48991|sc-99215|sc-393368|sc-393071 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human CYP2U1(457-473aa EKPEDFYPNRFLDDQGQ).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CYP2U1
Protein Name Cytochrome P450 2U1
Molecular Weight 61987 MW
Protein Function Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes. .
Tissue Specificity Widely expressed with stronger expression in thymus, heart and cerebellum. .
Subcellular Localization Endoplasmic reticulum membrane ; Multi-pass membrane protein . Microsome membrane .
Uniprot ID Q7Z449
Alternative Names Cytochrome P450 2U1;;CYP2U1;
Research Areas |cardiovascular|lipids / lipoproteins|lipid metabolism|cytochromes| signal transduction| cardiovascular|fatty acids| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipases|mitochondrial metabolism|types of disease|cancer|redox metabolism|fatty acid oxidation|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cytochrome P450 2U1

CYP2U1(Cytochrome P450, Family 2, Subfamily U, Polypeptide 1), is a protein that in humans is encoded by the CYP2U1 gene. Members of the P450 enzyme family have roles in the tissue-specific conversion of substrates into locally active hormones, vitamins, and signaling molecules, including arachidonic acid derivatives known as eicosanoids. The International Radiation Hybrid Mapping Consortium mapped the CYP2U1 gene to chromosome 4.Chuang et al.(2004) stated that CYP2U1 maps to 4q25. Using recombination experiments in Sf9 insect cells, Chuang et al.(2004) found that CYP2U1 metabolized arachidonic acid, docosahexaenoic acid(DHA), and other long chain fatty acids. Chuang et al.(2004) suggested that CYP2U1 may play a role in brain and immune functions.

Anti-CYP2U1 Antibody Images

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Anti-CYP2U1 Antibody
Anti-CYP2U1 antibody, PA2022, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: MM453 Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: HT1080 Cell Lysate
Anti-CYP2U1 Antibody
Anti-CYP2U1 antibody, PA2022, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-CYP2U1 Antibody
Anti-CYP2U1 antibody, PA2022, ICC
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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.