Click image to see more details
-
-
-
-
-
+3
Product Info Summary
| SKU: | A04286 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HSP70 HSPA1L Monoclonal Antibody
SKU/Catalog Number
A04286
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HSP70 HSPA1L Monoclonal Antibody catalog # A04286. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP70 HSPA1L Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04286)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
3G1
Isotype
IgG1
Immunogen
Human protein HSP70 at C-terminal
Reactive Species
A04286 is reactive to HSPA1L in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
70.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04286 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-2000
IF 1:100-200
IHC 1:50-300
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.
Click image to see more details
Western blot analysis of 1) Hela, 2) Mouse Brain, diluted at 1:2000.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, HSP70 Monoclonal Antibody (3G10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, HSP70 Monoclonal Antibody (3G10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunofluorescence analysis of Hela cell. 1, AIM2 Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). HSP70 Monoclonal Antibody (3G10) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Click image to see more details
Immunofluorescence analysis of Mouse-lung tissue. 1, HSP70 Monoclonal Antibody (3G10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Click image to see more details
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Specific Publications For Anti-HSP70 HSPA1L Monoclonal Antibody (A04286)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HSP70 HSPA1L Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-HSP70 HSPA1L Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-HSP70 HSPA1L Monoclonal Antibody
Question
Would anti-HSP70 Monoclonal antibody A04286 work on monkey IF with sperm?
D. Huang
Verified customer
Asked: 2020-04-24
Answer
Our lab technicians have not tested anti-HSP70 Monoclonal antibody A04286 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-HSP70 Monoclonal antibody A04286 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey sperm in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this A04286 anti-HSP70 Monoclonal antibody reactive to the isotypes of HSPA1L?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The immunogen of A04286 anti-HSP70 Monoclonal antibody is Synthetic Peptide. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for sperm using anti-HSP70 Monoclonal antibody A04286. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-09
Question
Do you have a BSA free version of anti-HSP70 Monoclonal antibody A04286 available?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HSP70 Monoclonal antibody A04286 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-18
Question
Will A04286 anti-HSP70 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
You can see on the product datasheet, A04286 anti-HSP70 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-17
Question
See below the WB image, lot number and protocol we used for sperm using anti-HSP70 Monoclonal antibody A04286. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-04-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-30
Question
We are currently using anti-HSP70 Monoclonal antibody A04286 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
The anti-HSP70 Monoclonal antibody (A04286) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-08
Question
My question regarding product A04286, anti-HSP70 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04286 anti-HSP70 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-07
Question
I was wanting to use your anti-HSP70 Monoclonal antibody for WB for human sperm on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human sperm identification?
S. Lewis
Verified customer
Asked: 2017-11-03
Answer
As indicated on the product datasheet, A04286 anti-HSP70 Monoclonal antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human sperm in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-03
Question
I see that the anti-HSP70 Monoclonal antibody A04286 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-08-16
Question
Is a blocking peptide available for product anti-HSP70 Monoclonal antibody (A04286)?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
We do provide the blocking peptide for product anti-HSP70 Monoclonal antibody (A04286). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-26
Question
I am interested in to test anti-HSP70 Monoclonal antibody A04286 on human sperm for research purposes, then I may be interested in using anti-HSP70 Monoclonal antibody A04286 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Patel
Verified customer
Asked: 2015-01-06
Answer
The products we sell, including anti-HSP70 Monoclonal antibody A04286, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-01-06
Question
Would anti-HSP70 Monoclonal antibody A04286 work for WB with sperm?
J. Jones
Verified customer
Asked: 2013-07-16
Answer
According to the expression profile of sperm, HSPA1L is highly expressed in sperm. So, it is likely that anti-HSP70 Monoclonal antibody A04286 will work for WB with sperm.
Boster Scientific Support
Answered: 2013-07-16
