Anti-HSP70/HSPA1B Antibody

Product Name

Anti-HSP70/HSPA1B Antibody

View all HSPA1B Antibodies

SKU/Catalog Number

A30415

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-HSP70/HSPA1B Antibody catalog # A30415. Tested in WB, IHC, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Cite This Product

Anti-HSP70/HSPA1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30415)

Host

Rabbit

Contents

500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Immunogen

E.coli-derived human HSP70/HSPA1B recombinant protein (Position: Y294-E638).

Reactive Species

A30415 is reactive to HSPA1B in Human, Mouse, Rat

Calculated molecular weight

70.1 kDa

Background of HSPA1B

1. What is Hsp70/HSP1A? HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers. 2. FAQs for Hsp70 a. I cannot detect Hsp70 by western blotting HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control. a. What loading control can I use for cellular stress experiments with Hsp70? Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A30415 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
ImmunoPrecipitation, 1:250-300
ELISA, 1:100-1000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information HSPA1B

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