Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™

Boster Bio Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™ catalog # PB9089. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: PB9089
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™

See all HSP90AA1 products

SKU/Catalog Number

PB9089

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™ catalog # PB9089. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9089)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Hsp90 alpha recombinant protein (Position: P2-V365). Human Hsp90 alpha shares 99% amino acid (aa) sequence identity with both mouse and rat Hsp90 alpha.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9089 is reactive to HSP90AA1 in Human, Mouse, Rat

Applications

PB9089 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For HSP90AA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P07900

Gene ID

3320

Gene Name

HSP90AA1

Full Name

Heat shock protein HSP 90-alpha

Weight

84.66kDa

Superfamily

heat shock protein 90 family

Alternative Names

FLJ31884; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein HSP 90-alpha; HSP86; Hsp89; HSP89A; HSP90 alpha; Hsp90; HSP90A; HSP90AA1; HSP90AHSP86; HSP90N; HSPC1; HSPC1HSP 86; HSPCA; HSPCAHSP89A; HSPCAL1; HSPCAL4; HSPN; LAP2; Renal carcinoma antigen NY-REN-38 HSP90AA1 EL52, HEL-S-65p, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp103, Hsp89, Hsp90, LAP-2, LAP2 heat shock protein 90 alpha family class A member 1 heat shock protein HSP 90-alpha|HSP 86|LPS-associated protein 2|epididymis luminal secretory protein 52|epididymis secretory sperm binding protein Li 65p|heat shock 86 kDa|heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha|heat shock protein 90kDa alpha (cytosolic), class A member 1|heat shock protein 90kDa alpha family class A member 1|lipopolysaccharide-associated protein 2|renal carcinoma antigen NY-REN-38

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HSP90AA1, check out the HSP90AA1 Infographic

HSP90AA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HSP90AA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9089 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Biaoxue Rong,Youwen Zhang,Junye Wang et al.Increased STIP1 and Hsp90 Correlate with Progression and Prognosis of Lung Adenocarcinoma,23 February 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-226260/v1]
Species: Human
PB9089 usage in article: APP:IHC, SAMPLE:LAC TISSUE, DILUTION:1:300

Youwen Zhang,Junye Wang,Shucheng Ye et al.Co-overexpression of STIP1 and Hsp90 correlates with progression and prognosis of lung adenocarcinoma,29 January 2020,PREPRINT (Version 2) available at Research Square [https://doi.org/10.21203/rs.2.13680/v2]
Species: Human
PB9089 usage in article: APP:IHC, SAMPLE:LAC TISSUE, DILUTION:1:300

The Effect of 5?-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co ?-ray

Jiang Q, Wang Y, Li T, Shi K, Li Z, Ma Y, Li F, Luo H, Yang Y, Xu C. Mol Biol Cell. 2011 Apr 15;22(8):1167-80. Doi: 10.1091/Mbc.E10-10-0860. Epub 2011 Feb 23. Heat Shock Protein 90-Mediated Inactivation Of Nuclear Factor-??b Switches Autophagy To ...

Have you used Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™

Question

Is a blocking peptide available for product anti-Hsp90 alpha/HSP90AA1 antibody (PB9089)?

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

We do provide the blocking peptide for product anti-Hsp90 alpha/HSP90AA1 antibody (PB9089). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-19

Question

Will PB9089 anti-Hsp90 alpha/HSP90AA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

It shows on the product datasheet, PB9089 anti-Hsp90 alpha/HSP90AA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-20

Question

Does anti-Hsp90 alpha/HSP90AA1 antibody PB9089 work for WB with pituitary?

B. Taylor

Verified customer

Asked: 2019-08-13

Answer

According to the expression profile of pituitary, HSP90AA1 is highly expressed in pituitary. So, it is likely that anti-Hsp90 alpha/HSP90AA1 antibody PB9089 will work for WB with pituitary.

Boster Scientific Support

Answered: 2019-08-13

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pituitary using anti-Hsp90 alpha/HSP90AA1 antibody PB9089. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-25

Question

I was wanting to use to test anti-Hsp90 alpha/HSP90AA1 antibody PB9089 on human pituitary for research purposes, then I may be interested in using anti-Hsp90 alpha/HSP90AA1 antibody PB9089 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

The products we sell, including anti-Hsp90 alpha/HSP90AA1 antibody PB9089, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-31

Question

you antibody using your anti-Hsp90 alpha/HSP90AA1 antibody for regulation of protein ubiquitination studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

We appreciate your inquiry. This PB9089 anti-Hsp90 alpha/HSP90AA1 antibody is validated on intestinal cancer tissue, tissue lysate, mouse brain, brain tissue, rat liver tissue, hela whole cell lysate, raji whole cell lysate. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-04-17

Question

I have a question about product PB9089, anti-Hsp90 alpha/HSP90AA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-09-04

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9089 anti-Hsp90 alpha/HSP90AA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-04

Question

We have tried in the past anti-Hsp90 alpha/HSP90AA1 antibody for WB on placenta teratocarcinoma last year. I am using rat, and We want to use the antibody for ICC next. We are interested in examining placenta teratocarcinoma as well as middle temporal gyrus in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

Z. Wu

Verified customer

Asked: 2018-06-18

Answer

I have checked the website and datasheets of our anti-Hsp90 alpha/HSP90AA1 antibody and it seems that PB9089 has been tested on rat in both WB and ICC. Thus PB9089 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-06-18

Question

I see that the anti-Hsp90 alpha/HSP90AA1 antibody PB9089 works with WB, what is the protocol used to produce the result images on the product page?

A. Dhar

Verified customer

Asked: 2018-05-04

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-04

Question

We have observed staining in human placenta teratocarcinoma. Are there any suggestions? Is anti-Hsp90 alpha/HSP90AA1 antibody supposed to stain placenta teratocarcinoma positively?

Verified Customer

Verified customer

Asked: 2018-02-02

Answer

From literature placenta teratocarcinoma does express HSP90AA1. From Uniprot.org, HSP90AA1 is expressed in middle temporal gyrus, peripheral blood lymphocyte, placenta, placenta teratocarcinoma, brain, cajal-retzius cell fetal brain cortex, heart, renal cell carcinoma, lymphoblast, cervix carcinoma, pituitary, t-cell, platelet, liver, leukemic t-cell, melanoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2018-02-02

Question

Is this PB9089 anti-Hsp90 alpha/HSP90AA1 antibody reactive to the isotypes of HSP90AA1?

Verified Customer

Verified customer

Asked: 2018-01-15

Answer

The immunogen of PB9089 anti-Hsp90 alpha/HSP90AA1 antibody is E.coli-derived human Hsp90 alpha recombinant protein (Position: P2-V365). Human Hsp90 alpha shares 99% amino acid (aa) sequence identity with both mouse and rat Hsp90 alpha. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-15

Question

I was wanting to use your anti-Hsp90 alpha/HSP90AA1 antibody for WB for human pituitary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pituitary identification?

Verified Customer

Verified customer

Asked: 2017-11-27

Answer

It shows on the product datasheet, PB9089 anti-Hsp90 alpha/HSP90AA1 antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pituitary in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-27

Question

Is there a BSA free version of anti-Hsp90 alpha/HSP90AA1 antibody PB9089 available?

Verified Customer

Verified customer

Asked: 2017-11-02

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsp90 alpha/HSP90AA1 antibody PB9089 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-11-02

Question

See below the WB image, lot number and protocol we used for pituitary using anti-Hsp90 alpha/HSP90AA1 antibody PB9089. Please let me know if you require anything else.

K. Banerjee

Verified customer

Asked: 2017-10-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-05

Question

Our team were well pleased with the WB result of your anti-Hsp90 alpha/HSP90AA1 antibody. However we have observed positive staining in placenta nucleus. using this antibody. Is that expected? Could you tell me where is HSP90AA1 supposed to be expressed?

A. Li

Verified customer

Asked: 2015-08-07

Answer

Based on literature, placenta does express HSP90AA1. Generally HSP90AA1 expresses in nucleus. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2015-08-07

Question

We are currently using anti-Hsp90 alpha/HSP90AA1 antibody PB9089 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

D. Wu

Verified customer

Asked: 2014-09-11

Answer

The anti-Hsp90 alpha/HSP90AA1 antibody (PB9089) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-09-11

Conjugation

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
PB9089
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.