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Product Info Summary
| SKU: | A05636-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NARG1/NAA15 Antibody Picoband®
SKU/Catalog Number
A05636-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NARG1/NAA15 Antibody Picoband® catalog # A05636-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NARG1/NAA15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05636-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NARG1, different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A05636-1 is reactive to NAA15 in Human, Mouse, Rat
Observed Molecular Weight
101 kDa
Calculated molecular weight
101.3 kDa
Background of NAA15
NMDA receptor-regulated protein 1 (NARG1), also known as GA19 or Tbdn100 is a protein that in humans is encoded by the NAA15 gene. It is mapped to chromosome 4. NARG1 is the auxiliary subunit of the NatA (Nα-acetyltransferase A) complex. Both, Naa15 and Naa16 interact with the ribosome in yeast (via the ribosomal proteins, uL23 and uL29), humans and rat, thereby linking the NatA/Naa10 to the ribosome and facilitating co-translational acetylation of nascent polypeptide chains as they emerges from the exit tunnel. Furthermore, Naa15 might act as a scaffold for other factors, including the chaperone like protein HYPK (Huntingtin Interacting Protein K) and Naa50, the catalytic acetyltransferase subunit of NatE.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05636-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human Hela whole cell, human 293T whole ce, human K562 whole cel, mouse brain tissue, mouse heart tissue
IHC: human gastric cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of NARG1/NAA15 using anti-NARG1/NAA15 antibody (A05636-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARG1/NAA15 antigen affinity purified polyclonal antibody (A05636-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NARG1/NAA15 at approximately 101 kDa. The expected band size for NARG1/NAA15 is at 101 kDa.
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IHC analysis of NARG1/NAA15 using anti-NARG1/NAA15 antibody (A05636-1).
NARG1/NAA15 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NARG1/NAA15 Antibody (A05636-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-NARG1/NAA15 Antibody Picoband® (A05636-1)
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Customer Q&As
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3 Customer Q&As for Anti-NARG1/NAA15 Antibody Picoband®
Question
Does anti-NARG1/NAA15 antibody A05636-1 work for WB with heart osteoblast?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
According to the expression profile of heart osteoblast, NAA15 is highly expressed in heart osteoblast. So, it is likely that anti-NARG1/NAA15 antibody A05636-1 will work for WB with heart osteoblast.
Boster Scientific Support
Answered: 2019-07-03
Question
We are currently using anti-NARG1/NAA15 antibody A05636-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
D. Brown
Verified customer
Asked: 2019-03-13
Answer
The anti-NARG1/NAA15 antibody (A05636-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-13
Question
Please see the WB image, lot number and protocol we used for heart osteoblast using anti-NARG1/NAA15 antibody A05636-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-31
