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Product Info Summary
| SKU: | M01114-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, ICC |
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Product info
Product Name
Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody
SKU/Catalog Number
M01114-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody (Catalog# M01114-2). Tested in Flow Cytometry, ICC application(s). This antibody reacts with Human, Primate.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01114-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
24-31
Isotype
Mouse IgG1
Immunogen
Human CD154 fusion protein. The mouse monoclonal antibody 24-31 detects an extracellular epitope of CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Reactive Species
M01114-2 is reactive to CD40LG in Human, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
29.4 kDa
Background of CD40LG
CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01114-2 is guaranteed for Flow Cytometry, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2-6 μg/ml.
Validation Images & Assay Conditions
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Separation of human CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in flow cytometry analysis (surface staining) of stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) purified antibody (concentration in sample 2 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) purified antibody (concentration in sample 2 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody (M01114-2)
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