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We validate the specificity of these antibodies to CD40 ligand by testing them on tissues known to express CD40LG positively and negatively. Browse below to find the CD40LG antibody that suites your experiment. We have 16 of these antibodies and many publications and validation images.
If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.
Facts about CD40 ligand.
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 together with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T- cells.
|tumor necrosis factor family|
CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5
|Sequence:||X; NC_000023.11 (136648158..136660390)|
Specifically expressed on activated CD4+ T- lymphocytes.
Cell membrane; Single-pass type II membrane protein. Cell surface.; [CD40 ligand, soluble form]: Secreted. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Boster Bio's CD40LG ELISA Kit is a product you may have heard about. If you aren't sure what it is, or what you can be expecting from it, keep reading to learn more about how to achieve the best results with Boster Bio's CD40LG marker. You can also download a complimentary demo of the CD40LG ELISA Kit to see how it works.
Boster Bio's anti-CD40L/CD40LG marker is a specific rat anti-CD40LG marker. The antibody reacts to both Human and Rat sequences. It is stored at 20°C. The rats' sequence is more immune-friendly than the human one. This will allow you to identify the right cells to analyze.
The CD40LG ELISA Kit from Boster Bio is designed to detect the level of expression of this biomarker across a large range of samples. This antibody kit is sensitive to a picogram level. Boster Bio's products are validated against more than 250 cell types and tissues, resulting in high specificity. The kit can be purchased through tebu.bio
The kit comes with 96-well plates that can be used for testing in 96. This antibody is able to detect the concentrations of Mouse CD40lg within supernatant cells from cell culture. The Kit utilizes a microplate reader that measures absorbance at the 450nm wavelength to determine the concentration of this protein. Boster Pipettes can be used to disperse 0.5 ul through 1 milliliter volumes. Boster Bio's CD40LGELISA Kit can also be available for custom research. It comes with product credits that can be shared with others.
Boster Bio's CD40L ELISA Kit is a sandwich-based enzyme-linked immunosorbent assay. The monoclonal mouse CD40L antibodies have been precoated onto 96 well plates. Test samples and standards are then added. A goat biotinylated detection polyclonal antibody is then added. The test samples and the standards are washed out to remove conjugates that are not bound. The HRP enzyme reaction can be observed by placing TMB substrate onto the plate and observing the TMB color.
Boster Bio's CD40LG ELIISA kit is compatible with mouse spleenocytes and PBMCs. CD40 Ligand antibodies can be used in a variety applications and have been validated in the field of Flow Cytometry and Cell Activation. There are more than 40 vendors of anti-CD40L antibodies. There are anti-CD40L antibodies human PBMCs, mouse spleenocytes, and Chimpanzee PBMs.
*More publications can be found for each product on its corresponding product page