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Product Info Summary
| SKU: | M05818 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD157 Purified BST1 Monoclonal Antibody
SKU/Catalog Number
M05818
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD157 Purified BST1 Monoclonal Antibody (Catalog# M05818). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD157 Purified BST1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05818)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SY11B5
Isotype
Mouse IgG1 kappa
Immunogen
Human CD157. The mouse monoclonal antibody SY11B5 recognizes CD157, an approximately 45 kDa GPI-anchored extracellular protein expressed mainly on monocytes, macrophages, granulocytes and bone marrow stromal cells.
Cross-reactivity
This antibody weakly cross-reacts with murine SIT.
Reactive Species
M05818 is reactive to BST1 in Human, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
35.7 kDa
Background of BST1
CD157 (cADPr hydrolase 2) is a GPI-anchored ectoenzyme possessing ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activity. It uses NAD and cADP-ribose as substrates. CD157 is expressed on granulocytes, monocytes, macrophages, follicular dendritic cells, bone marrow stromal cells and human umbilical cord vein endothelial cells. In case of rheumatoid arthritis is expression is often higher and it is also differentially expressed in the myeloid leukemias. It may also have a signaling role.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05818 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: Non-reducing conditions.
Immunohistochemistry: 5-10 μg/ml.
Flow cytometry: 1-12 µg/ml
Validation Images & Assay Conditions
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Separation of human neutrophil granulocytes (red-filled) from CD157 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD157 (SY11B5) purified antibody (concentration in sample 4 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD157 (SY11B5) purified antibody (concentration in sample 4 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD157 Purified BST1 Monoclonal Antibody (M05818)
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