Anti-NMI Antibody Picoband™ (monoclonal, 2F3)

N myc interactor antibody

Boster Bio Anti-NMI Antibody Picoband™ (monoclonal, 2F3) catalog # M02768. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M02768
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-NMI Antibody Picoband™ (monoclonal, 2F3)

View all N myc interactor Antibodies

SKU/Catalog Number

M02768

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NMI Antibody Picoband™ (monoclonal, 2F3) catalog # M02768. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NMI Antibody Picoband™ (monoclonal, 2F3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02768)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

2F3

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M02768 is reactive to NMI in Human, Mouse, Rat

Applications

M02768 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Background of N myc interactor

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For NMI (Source: Uniprot.org, NCBI)

Uniprot ID

Q13287

Gene ID

9111

Gene Name

NMI

Full Name

N-myc-interactor

Weight

35.057kDa

Superfamily

NMI family

Alternative Names

Nmi; N-myc (and STAT) interactor; N-myc and STAT interactor; N-myc interactor; N-myc-interactor NMI N-myc and STAT interactor N-myc-interactor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NMI, check out the NMI Infographic

NMI infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NMI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

No publications found for M02768

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5 Customer Q&As for Anti-NMI Antibody Picoband™ (monoclonal, 2F3)

Question

We want to test anti-NMI antibody (monoclonal, 2F3) M02768 on rat bone marrow for research purposes, then I may be interested in using anti-NMI antibody (monoclonal, 2F3) M02768 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

The products we sell, including anti-NMI antibody (monoclonal, 2F3) M02768, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-13

Question

Is a blocking peptide available for product anti-NMI antibody (monoclonal, 2F3) (M02768)?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

We do provide the blocking peptide for product anti-NMI antibody (monoclonal, 2F3) (M02768). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-13

Question

Is there a BSA free version of anti-NMI antibody (monoclonal, 2F3) M02768 available?

Verified Customer

Verified customer

Asked: 2018-10-31

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NMI antibody (monoclonal, 2F3) M02768 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-10-31

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-NMI antibody (monoclonal, 2F3) M02768. Let me know if you need anything else.

B. Kulkarni

Verified customer

Asked: 2016-12-26

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-12-26

Question

I was wanting to use your anti-NMI antibody (monoclonal, 2F3) for IHC-P for rat bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat bone marrow identification?

A. Mangal

Verified customer

Asked: 2015-06-11

Answer

It shows on the product datasheet, M02768 anti-NMI antibody (monoclonal, 2F3) has been tested for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat bone marrow in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-06-11

Size

Conjugation

Total: $315

SKU:M02768

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M02768
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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