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Product Info Summary
| SKU: | M02009 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC |
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Product info
Product Name
Anti-Hu CD255 Purified TNFSF12 Monoclonal Antibody
SKU/Catalog Number
M02009
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD255 Purified TNFSF12 Monoclonal Antibody (Catalog# M02009). Tested in Flow Cytometry, IHC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD255 Purified TNFSF12 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02009)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
CARL-1
Isotype
Mouse IgG3 kappa
Immunogen
Human CD255-transfected 2PK-3 cells. The mouse monoclonal antibody CARL-1 recognizes an extracellular epitope of CD255 / TWEAK, a type II transmembrane protein of the TNF superfamily able to induce apoptosis weakly in many cell types.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02009 is reactive to TNFSF12 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
27.2 kDa
Background of TNFSF12
CD255 / TWEAK (TNF-related weak inducer of apoptosis), a type II transmembrane protein expressed as membrane-bound and secreted form, can induce apoptosis in many tissues and cell lines through its receptor CD266 / TWEAK R. On the other hand, in endothelial cells this interaction can induce proliferation and promote angiogenesis including neovascularization of tumours. CD255 can act in a juxtacrine manner to initiate cellular responses, and induces secretion of pro-inflammatory cytokines. Besides CD266, CD255 may also bind to DR3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02009 is guaranteed for Flow Cytometry, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Separation of CD255 transfected l5178Y cells (red-filled) from CD255 nontransfected (WT) l5178Y cells (black-dashed) in flow cytometry analysis (surface staining) of l5178Y cell suspensions stained using anti-human CD255 (CARL-1) purified antibody (concentration in sample 3 µg/ml) GAM PE.
Specific Publications For Anti-Hu CD255 Purified TNFSF12 Monoclonal Antibody (M02009)
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