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Product Info Summary
| SKU: | M00360-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Pig, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD47 Purified Monoclonal Antibody
SKU/Catalog Number
M00360-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD47 Purified Monoclonal Antibody (Catalog# M00360-1). Tested in Flow Cytometry, WB, IHC-F application(s). This antibody reacts with Primate, Human, Pig.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD47 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00360-1)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-122
Isotype
Mouse IgM
Immunogen
COS-7 (African green monkey) cells. The antibody MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
Reactive Species
M00360-1 is reactive to CD47 in Human, Pig, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
35.2 kDa
Background of CD47
CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00360-1 is guaranteed for Flow Cytometry, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Western blotting: Non-reducing conditions.
Validation Images & Assay Conditions
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Flow cytometry (surface staining) of lysed and washed porcine peripheral blood with purified anti-CD47 (MEM-122) (detection by anti-mouse IgM FITC).
Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03)
Panel B - porcine PBMC sample stained with anti-CD47 (MEM-122)
Cells in the granulocyte gate were used for analysis.
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Separation of human lymphocytes (red-filled) from human CD47 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD47 (MEM-122) purified antibody (concentration in sample 4 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD47 (MEM-122) purified antibody (concentration in sample 4 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD47 Purified Monoclonal Antibody (M00360-1)
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