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Product Info Summary
| SKU: | M01898 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Cow, Ferret, Human, Rabbit |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, WB |
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Product info
Product Name
Anti-Hu CD88 Purified C5AR1 Monoclonal Antibody
SKU/Catalog Number
M01898
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD88 Purified C5AR1 Monoclonal Antibody (Catalog# M01898). Tested in Flow Cytometry, IP, WB, IHC-P application(s). This antibody reacts with Rabbit, Ferret, Cow, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD88 Purified C5AR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01898)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
S5/1
Isotype
Mouse IgG2a kappa
Immunogen
Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR. The mouse monoclonal antibody S5/1 recognizes an extracellular epitope of CD88 protein, a 43 kDa receptor of C5a component of the complement cascade.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M01898 is reactive to C5AR1 in Cow, Ferret, Human, Rabbit
Observed Molecular Weight
42 kDa
Calculated molecular weight
39.3 kDa
Background of C5AR1
CD88 / C5aR is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01898 is guaranteed for Flow Cytometry, IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 3-12 μg/ml.
Validation Images & Assay Conditions
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Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD88 (S5/1) purified antibody (concentration in sample 3 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD88 (S5/1) purified antibody (concentration in sample 3 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD88 Purified C5AR1 Monoclonal Antibody (M01898)
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