Anti-Human CCNE1 Antibody

Western blot analysis of CCNE1 using anti-CCNE1 antibody (DZ41310).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HaCaT whole cell lysates,
Lane 2: human A2780cp20 whole cell lysates,
Lane 3: human HCT whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNE1 antigen affinity purified polyclonal antibody (DZ41310) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Anti-rabbit/mouse IgG horseradish peroxidase-conjugated at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Biorad Chemidoc system. A specific band was detected for CCNE1 at approximately 50 kDa. The expected band size for CCNE1 is at 47 kDa.

IF analysis of CCNE1 using anti-CCNE1 antibody (DZ41310).
CCNE1 was detected in an immunocytochemical section of HaCaT cells. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. And then incubated with 1:100-1:200 rabbit anti-CCNE1 Antibody (DZ41310) overnight at 4°C. Goat anti-rabbit IgG conjugated to Alexa Fluor 488 was used as secondary antibody at 1:1000 dilution and incubated for 1-2 hours at RT. The section was counterstained with DAPI. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.