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CCNE1 antibody (DZ41310) performs well in IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of CCNE1 using anti-CCNE1 antibody (DZ41310).
CCNE1 was detected in an immunocytochemical section of HaCaT cells. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. And then incubated with 1:100-1:200 rabbit anti-CCNE1 Antibody (DZ41310) overnight at 4°C. Goat anti-rabbit IgG conjugated to Alexa Fluor 488 was used as secondary antibody at 1:1000 dilution and incubated for 1-2 hours at RT. The section was counterstained with DAPI. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.
CCNE1 was detected in an immunocytochemical section of HaCaT cells. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. And then incubated with 1:100-1:200 rabbit anti-CCNE1 Antibody (DZ41310) overnight at 4°C. Goat anti-rabbit IgG conjugated to Alexa Fluor 488 was used as secondary antibody at 1:1000 dilution and incubated for 1-2 hours at RT. The section was counterstained with DAPI. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.
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Anti-Human CCNE1 Antibody
CCNE1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IF, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1:1000
ICC/IF: 1:100-1:200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Human CCNE1 Antibody
View all CCNE1 Antibodies
View all CCNE1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Calculated Molecular Weight
47.1 kDa
Cite This Product
Anti-Human CCNE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41310)
Review
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2 Reviews For Anti-Human CCNE1 Antibody
A strong nuclear signal for Cyclin E was observed in proliferating cells by IF. Results were reproducible and matched the expected patterns.
| SKU | DZ41310 |
|---|---|
| Application | Immunofluorescence |
| Sample | Human HaCaT cell |
| Sample Processing Description | Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked in 1% BSA for 1 hour before incubation with the primary antibody. |
| Primary Antibody | Human CCNE1 Antibody |
| Primary Incubation | 1:100-1:200, overnight at 4 ℃ |
| Secondary Antibody | Goat anti-rabbit IgG conjugated to Alexa Fluor 488 |
| Secondary Incubation | 1:1000, 1-2 hours in room temperature |
| Other Reagents used | PBS, 0.1% Triton X-100, 1% BSA, DAPI for nuclear counterstaining |
| Detection | Fluorescence microscopy using AlexaFluor488 (excitation 488 nm, emission 519 nm) using Zeiss LSM 900 Confocal Microscope with Airyscan 2 |
| Results Summary | The CyclinE-N15 antibody (DZ41310) worked fine in IF application using human cell lines. In IF, the nuclear localization of Cyclin E matched the known expression pattern. A fluorescent secondary antibody (Alexa Fluor 488) was used for detection and gave clear signal. Overall, a reliable antibody for D15 transcript Cyclin E detection in human cell systems. |
Kasturi Mitra
Verified customer
Submitted 2025-11-05
Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns.
| SKU | DZ41310 |
|---|---|
| Application | Western Blot |
| Sample | Human HaCaT cell, Human A2780 cell, Human HCT cell |
| Sample Processing Description | Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting |
| Primary Antibody | Human CCNE1 Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | Anti-rabbit/mouse IgG horseradish peroxidase-conjugated |
| Secondary Incubation | 1:10000, 1-2 hours in room temperature |
| Detection | Biorad Chemidoc |
| Results Summary | Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns. |
Kasturi Mitra
Verified customer
Submitted 2025-11-05
Product Promise
We promise Anti-Human CCNE1 Antibody performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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DZ41310
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026