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We validate the specificity of these antibodies to G1/S-specific cyclin-E1 by testing them on tissues known to express CCNE1 positively and negatively. Browse below to find the CCNE1 antibody that suites your experiment. We have 14 of these antibodies and many publications and validation images.
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Facts about G1/S-specific cyclin-E1.
CCNE; CCNE1; CCNEcyclin Es; Cyclin E1; cyclin Et; G1/S-specific cyclin-E1
|Sequence:||19; NC_000019.10 (29811991..29824312)|
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
As a biologist, you may be interested in the uses of the CCNE1 marker in cell-culture research. Boster Bio's CCNE1 antibody may be kept at -20°C during 1 year or at a temperature of 4°C for a month. This antibody is available as a lyophilized product in 20mM PBS containing 50 glycerol, 0.02% NaN and 0.022 percent NaN3. It is validated on western blot (WB) and has excellent cross-reactivity with Human.
The CCNE1 marker is used to determine the expression of a specific gene within human cells. It is a new marker that is able to detect brain tumors, gliomas, and multiple sclerosis. Its target gene is the CCNE1 protein. We first amplified the 3'-UTRs from this gene from human peripheral blood to measure its expression in human cells. Next, we inserted these fragments into the pGL3-promoter vector and then performed the qRT-PCR. We also tested the expression of a specific gene by using 2-DDCT and primers for RAF1 and miR-370-3p. We validated the CCNE1 expression using the WB method and discovered that the CCNE1 proteins was abundantly expressed in all cell lines.
CCNE1 expression is also associated with poor prognosis in the glioma. This gene regulates the cycle of cells in various kinds of cells. It is also believed that it can influence cell proliferation and cell-cycle arrest. This gene was identified to be targeted in a direct manner using an test of luciferase. Similar to this it was found that the activity of luciferase diminished when miR-497 was expressed too much.
Boster Bio's TUSC1 gene relates to the gene that encodes a protein with the same name. Scientists are able to submit results for specific species or application using this gene. Boster scientists also earn product credits for their results. These programs are open to all scientists around the globe. These programs permit researchers to present their findings to various research objectives for example, testing the effects of different drugs and therapies. To find out more about TUSC1 check out the Boster Bio website.
This antibody recognizes the CCNE1 genes in mammalian cells. The company was established in 1993 by Steven Xia. Boster Bio is a pioneer in the creation and production of highly sensitive antibodies that have high specificity. Boster Bio has spent over two decades perfecting its methods and technology. Their products are featured in more than 29,000 scientific publications. The company's CCNE1 monoclonal anti-mouse antibody has been thoroughly verified by Western blot, ELISA and Flow Cymetry.
The miR-497 binding protein binds to the 3'-UTR of the CCNE1 gene and inhibits cell proliferation as well as cell cycle arrest. Additionally, miR-497 blocks cell cycle progression by targeting the CCNE1 gene. CCNE1 is an important regulator of the cell cycle and the overexpression of the gene blocks this inhibitory effect. This inhibitory effect is known as CCNE1-inhibitory.
The CCNE1 gene regulates miR-497 expression. This gene has two roles as an oncogene, as well as suppressor. It is involved with cell communication, and a lot of its targets have cancer-related connections. It is linked to various biological processes, such as cell proliferation, invasion and resistance to therapeutics, in the glioma. It has also been linked with non-small cell lung cancer as well as cervical cancer.
Cellular growth is influenced by the CCNE1 gene. It plays a significant role in the growth and expansion of cancer cells. It is often activated in apoptosis and tumorigenesis. This makes it useful for studies on cancer cells. Boster Bio's CCNE1 gene-expression kit is a fantastic choice for a variety of applications.
CCNE1 expression in patients with cancer is associated with survival time. The RT-qPCR analysis also revealed that CCNE1 gene overexpression is linked with cell proliferation as well as cell cycle development. Furthermore, MTT assays and flow cytometry analysis revealed that the overexpression of the CCNE1 gene decreased the proliferation of glioma cells in lines and the expression of CCNE1 inhibited the growth of HCN-2 tumor cells.
It has been identified in liver and gastric cancers as a tumor suppressor, and also an oncogenic molecule. The highest levels of it were associated with longer survival for patients suffering from primary glioma. However, low levels of the gene were associated with better prognosis. The CCNE1 gene was discovered to be in direct correlation with the miR-486 gene expression levels which is a tumor suppressor.
*More publications can be found for each product on its corresponding product page