Anti-D-dopachrome decarboxylase DDT Antibody

DDT antibody

Boster Bio Anti-D-dopachrome decarboxylase DDT Antibody catalog # RP1011. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: RP1011
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-D-dopachrome decarboxylase DDT Antibody

View all DDT Antibodies

SKU/Catalog Number

RP1011

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-D-dopachrome decarboxylase DDT Antibody catalog # RP1011. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-D-dopachrome decarboxylase DDT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1011)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human DDT recombinant protein (Position: M1-L118).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1011 is reactive to DDT in Human, Mouse, Rat

Applications

RP1011 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

13-15 kDa

Calculated molecular weight

12.712kDa

Background of DDT

DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human,

Validation Images & Assay Conditions

Gene/Protein Information For DDT (Source: Uniprot.org, NCBI)

Gene Name

DDT

Full Name

D-dopachrome decarboxylase

Weight

12.712kDa

Superfamily

MIF family

Alternative Names

DDCT; D-Dopachrome Decarboxylase; D-dopachrome tautomeraseDDCT; DDT; DOPD; EC 4.1.1.84; MIF2; Phenylpyruvate Tautomerase II DDT D-DT, DDCT, MIF-2, MIF2 D-dopachrome tautomerase D-dopachrome decarboxylase|phenylpyruvate tautomerase II

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DDT, check out the DDT Infographic

DDT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DDT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-D-dopachrome decarboxylase DDT Antibody

Question

Is this RP1011 anti-DDT antibody reactive to the isotypes of DDT?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

The immunogen of RP1011 anti-DDT antibody is E. coli-derived human DDT recombinant protein(Position: M1-L118). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-04

Question

Is there a BSA free version of anti-DDT antibody RP1011 available?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DDT antibody RP1011 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-17

Question

We are currently using anti-DDT antibody RP1011 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

The anti-DDT antibody (RP1011) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-28

Question

Please see the WB image, lot number and protocol we used for placenta using anti-DDT antibody RP1011. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-30

Question

I have a question about product RP1011, anti-DDT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1011 anti-DDT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-23

Question

We are interested in to test anti-DDT antibody RP1011 on human placenta for research purposes, then I may be interested in using anti-DDT antibody RP1011 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-01-30

Answer

The products we sell, including anti-DDT antibody RP1011, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-01-30

Question

Does RP1011 anti-DDT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Gonzalez

Verified customer

Asked: 2016-04-26

Answer

It shows on the product datasheet, RP1011 anti-DDT antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-04-26

Order DetailsPrice
RP1011

100μg

$370
RP1011-10ug

10μg sample (liquid)

$99
RP1011-Biotin

100 μg Biotin conjugated

$570
RP1011-Cy3

100 μg Cy3 conjugated

$570
RP1011-Dylight488

100 μg Dylight488 conjugated

$570
RP1011-Dylight550

100 μg Dylight550 conjugated

$570
RP1011-Dylight594

100 μg Dylight594 conjugated

$570
RP1011-FITC

100 μg FITC conjugated

$570
RP1011-HRP

100 μg HRP conjugated

$570
RP1011-APC

100 μg APC conjugated

$670
RP1011-PE

100 μg PE conjugated

$670
RP1011-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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RP1011
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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