Anti-IFN gamma Antibody

Boster Bio Anti-IFN gamma Antibody catalog # RP1002. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 5 publication(s).

Product Info Summary

SKU: RP1002
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-IFN gamma Antibody

See all IFN-gamma products

SKU/Catalog Number

RP1002

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IFN gamma Antibody catalog # RP1002. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IFN gamma Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1002)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived human IFN gamma recombinant protein (Position: Q24-Q166).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1002 is reactive to IFNG in Human

Applications

RP1002 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For IFNG (Source: Uniprot.Org, NCBI)

Uniprot ID

P01579

Gene ID

3458

Gene Name

IFNG

Full Name

Interferon gamma

Weight

19.348kDa

Superfamily

type II (or gamma) interferon family

Alternative Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IFNG, check out the IFNG Infographic

IFNG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IFNG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1002 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface

Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation

Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion

Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

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Find answers in Q&As, reviews.

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4 Customer Q&As for Anti-IFN gamma Antibody

Question

My team were well pleased with the WB result of your anti-IFN gamma antibody. However we have seen positive staining in leukocyte secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

According to literature, leukocyte does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-11

Question

We have seen staining in human blood. What should we do? Is anti-IFN gamma antibody supposed to stain blood positively?

R. Jha

Verified customer

Asked: 2017-03-08

Answer

From what I have seen in literature blood does express IFNG. From what I have seen in Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-03-08

Question

We are currently using anti-IFN gamma antibody RP1002 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?

S. Krishna

Verified customer

Asked: 2016-06-17

Answer

The anti-IFN gamma antibody (RP1002) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-06-17

Question

We purchased anti-IFN gamma antibody for WB on leukocyte in a previous experiment. I am using human, and We want to use the antibody for IHC next. I would like examining leukocyte as well as blood in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

S. Jones

Verified customer

Asked: 2013-07-15

Answer

I looked at the website and datasheets of our anti-IFN gamma antibody and it appears that RP1002 has been validated on human in both WB and IHC. Thus RP1002 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-07-15

Conjugation

Size

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RP1002
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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