Human IFN Gamma ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IFN gamma. 96wells/kit, with removable strips. Cited in 20 publication(s). Independently reviewed in 1 review(s).
Human IFN Gamma ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates and serum.|
|Sample Volume:||100μl per well|
|Product Name||Human IFN Gamma ELISA Kit PicoKine™
See all IFNG primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Ifn Gamma/IFNG with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Ifn Gamma/IFNG tissue specificity: Released primarily from activated T lymphocytes.|
|Cite This Product||Human IFN Gamma ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0373)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates and serum.|
|Immunogen||Expression system for standard: E.coli; Immunogen sequence: Q24-Q166|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Interferon gamma|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Human IFN Gamma ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0373-CAP||96-well plate precoated with anti-Human IFNG antibody||1|
|EK0373-ST||lyophilized recombinant Human IFNG standard||10ng/tube|
|EK0373-DA||biotinylated anti-Human IFNG antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0373 Human IFN Gamma ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IFN Gamma ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human IFN Gamma ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 20
|Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.||PubMed ID 24656779|
|Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...||PubMed ID 23423691|
|Zhang L1, Kong D, Meng H, Han C, Zhu J, Qiao J, He Y, Wang T, Li X, Zhang F, Jin X. Cell Physiol Biochem. 2016;40(6):1473-1486. doi: 10.1159/000453199. Epub 2016 Dec 20. Plasma Gelsolin Promotes Proliferation of Mesangial Cell in IgA Nephropathy||PubMed ID 27997897|
|Li J, Liu X, Duan Y, Wang H, Su W, Wang Y, Zhuang G, Fan Y. Oncol Lett. 2018 Mar;15(3):3496-3503. doi: 10.3892/ol.2018.7786. Epub 2018 Jan 12. Abnormal expression of circulating and tumor-infiltrating carcinoembryonic antigen-related cell adhesion...||PubMed ID 29467871|
|Adenovirus-mediated delivery of interferon-? gene inhibits the growth of nasopharyngeal carcinoma||PubMed ID 23272637|
|Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages||PubMed ID 25861639|
|A novel anti-GD2/4-1BB chimeric antigen receptor triggers neuroblastoma cell killing||PubMed ID 26298772|
|Effects of Probiotics on Biomarkers of Oxidative Stress and Inflammatory Factors in Petrochemical Workers: A Randomized, Double-blind, Placebo-controlled Trial||PubMed ID 26445629|
|Epigenetic suppression of the antitumor cytotoxicity of NK cells by histone deacetylase inhibitor valproic acid||PubMed ID 27152238|
|Cytokine Pattern is Affected by Training Intensity in Women Futsal Players||PubMed ID 27162527|
|Capsaicin induces immunogenic cell death in human osteosarcoma cells||PubMed ID 27446273|
|MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma||PubMed ID 27494859|
|Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion||PubMed ID 27698705|
|??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function||PubMed ID 28259946|
|Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis||PubMed ID 26673286|
|Psoriasis 1'reduces psoriasis%u2011like skin inflammation by inhibiting the VDR%u2011mediated nuclear NF%u2011%u03BAB and STAT signaling pathways||PubMed ID 30015892|
|The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility||PubMed ID 29914245|
|Small molecule GL-V9 protects against colitis-associated colorectal cancer by limiting NLRP3 inflammasome through autophagy||PubMed ID 29296531|
|A novel derivative of (-) mycousnine produced by the endophytic fungus Mycosphaerella nawae, exhibits high and selective immunosuppressive activity on T %u2026||PubMed ID 28725220|
|The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells||PubMed ID 30010837|
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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