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Product Info Summary
| SKU: | RP1014 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Interleukin-10 IL10 Antibody Picoband®
SKU/Catalog Number
RP1014
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1014. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-10 IL10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1014)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL-10 recombinant protein (Position: S19-N178).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1014 is reactive to IL10 in Human
Observed Molecular Weight
17 kDa
Calculated molecular weight
20.5 kDa
Background of IL10
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1014 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: recombinant human IL10 protein
IHC: human appendix tissue, human spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IL10 using anti-IL10 antibody (RP1014).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant human IL10 protein 10 ng,
Lane 2: recombinant human IL10 protein 5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (RP1014) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL10 at approximately 17 kDa.
Click image to see more details
IHC analysis of IL10 using anti-IL10 antibody (RP1014).
IL10 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL10 Antibody (RP1014) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of IL10 using anti-IL10 antibody (RP1014).
IL10 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL10 Antibody (RP1014) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Interleukin-10 IL10 Antibody Picoband® (RP1014)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Interleukin-10 IL10 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody RP1014. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-05
Question
Does RP1014 anti-IL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
As indicated on the product datasheet, RP1014 anti-IL10 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-28
Question
Is this RP1014 anti-IL10 antibody reactive to the isotypes of IL10?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The immunogen of RP1014 anti-IL10 antibody is E. coli-derived human IL-10 recombinant protein(Position: S19-N178). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-14
Question
We have observed staining in human t-cell. What should we do? Is anti-IL10 antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
From what I have seen in literature t-cell does express IL10. From what I have seen in Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2020-02-12
Question
We are currently using anti-IL10 antibody RP1014 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The anti-IL10 antibody (RP1014) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-10
Question
Our lab were happy with the WB result of your anti-IL10 antibody. However we have been able to see positive staining in esophagus secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
According to literature, esophagus does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2019-10-30
Question
I see that the anti-IL10 antibody RP1014 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-04
Question
Will anti-IL10 antibody RP1014 work for ELISA with esophagus?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
According to the expression profile of esophagus, IL10 is highly expressed in esophagus. So, it is likely that anti-IL10 antibody RP1014 will work for ELISA with esophagus.
Boster Scientific Support
Answered: 2019-05-17
Question
I was wanting to use to test anti-IL10 antibody RP1014 on human esophagus for research purposes, then I may be interested in using anti-IL10 antibody RP1014 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Johnson
Verified customer
Asked: 2018-10-05
Answer
The products we sell, including anti-IL10 antibody RP1014, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-05
Question
Please see the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody RP1014. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-23
Question
Our lab used your anti-IL10 antibody for ELISA on t-cell in a previous experiment. I am using human, and We are going to use the antibody for WB next. I am interested in examining t-cell as well as esophagus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
I looked at the website and datasheets of our anti-IL10 antibody and it seems that RP1014 has been validated on human in both ELISA and WB. Thus RP1014 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-07-12
Question
Is a blocking peptide available for product anti-IL10 antibody (RP1014)?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
We do provide the blocking peptide for product anti-IL10 antibody (RP1014). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-31
Question
I was wanting to use your anti-IL10 antibody for ELISA for human esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human esophagus identification?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
You can see on the product datasheet, RP1014 anti-IL10 antibody has been tested for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-09
Question
Can you help my question with product RP1014, anti-IL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
H. Yang
Verified customer
Asked: 2017-08-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1014 anti-IL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-02
Question
Do you have a BSA free version of anti-IL10 antibody RP1014 available?
R. Brown
Verified customer
Asked: 2017-02-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL10 antibody RP1014 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-02-21
