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Product Info Summary
| SKU: | M00102-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA (Cap) |
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Product info
Product Name
Anti-human IL6 Antibody (monoclonal, 7D12)
SKU/Catalog Number
M00102-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-human IL6 Antibody (monoclonal, 7D12) catalog # M00102-2. Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human IL6 Antibody (monoclonal, 7D12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00102-2)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
7D12
Isotype
Mouse IgG2a
Immunogen
sf21-derived human IL6 recombinant protein (Position: P29-M212).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00102-2 is reactive to IL6 in Human
Calculated molecular weight
23.7 kDa
Background of IL6
Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al. (1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) mapped the IL-6 gene to chromosome 7p15.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00102-2 is guaranteed for ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (Cap), 1-5μg/ml, Human
Validation Images & Assay Conditions
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Specific Publications For Anti-human IL6 Antibody (monoclonal, 7D12) (M00102-2)
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4 Customer Q&As for Anti-human IL6 Antibody (monoclonal, 7D12)
Question
Our team were content with the WB result of your anti-human IL6 antibody (monoclonal, 7D12). However we have been able to see positive staining in left coronary artery secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
According to literature, left coronary artery does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-13
Question
Hi Support: I purchased the antibody against IL-6 with the SKU M00102-2 and used it as primary antibody in Western Blot. However, my results obtained 8 colored bands on the membrane (24 to 150kD), in which only one of them corresponds to the right molecular weight (about 24 kD). I want to figure out whether it is non-specific binding or not. Have you encountered the same profile before? Do you know which site of IL-6 is targeted by this antibody SKU M00102-2?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
The mature protein of IL-6 can be highly N-glycosylated so the actual band is much higher than the expected. The sizes of the bands depend on the complexity of the polysaccharide chain. May I know what protocol you used in Western blot, to see if any alterations/optimizations are necessary?
Boster Scientific Support
Answered: 2019-10-14
Question
We are currently using anti-human IL6 antibody (monoclonal, 7D12) M00102-2 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-human IL6 antibody (monoclonal, 7D12) (M00102-2) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
We have observed staining in human left coronary artery. Do you have any suggestions? Is anti-human IL6 antibody (monoclonal, 7D12) supposed to stain left coronary artery positively?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
From what I have seen in literature left coronary artery does express IL6. From what I have seen in Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-24
