Anti-human IL6 Antibody Picoband™ (monoclonal, 7D12)

Boster Bio Anti-human IL6 Antibody Picoband™ (monoclonal, 7D12) catalog # M00102-2. Tested in ELISA applications. This antibody reacts with Human. Cited in 11 publication(s).

Product Info Summary

SKU: M00102-2
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: ELISA

Product Name

Anti-human IL6 Antibody Picoband™ (monoclonal, 7D12)

See all IL-6 products

SKU/Catalog Number

M00102-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-human IL6 Antibody Picoband™ (monoclonal, 7D12) catalog # M00102-2. Tested in ELISA applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human IL6 Antibody Picoband™ (monoclonal, 7D12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00102-2)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

7D12

Isotype

IgG2a

Immunogen

sf21-derived human IL6 recombinant protein (Position: P29-M212).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00102-2 is reactive to IL6 in Human

Applications

M00102-2 is guaranteed for ELISA Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA (Cap), 1-5μg/ml, Human

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For IL6 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05231

Gene ID

3569

Gene Name

IL6

Full Name

Interleukin-6

Weight

23.718kDa

Superfamily

IL-6 superfamily

Alternative Names

B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL6, check out the IL6 Infographic

IL6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00102-2 has been cited in 11 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Guo L,Jiang B,Li D,Xiao X. Nephroprotective Effect of Adropinin Against Streptozotocin-Induced Diabetic Nephropathy in Rats: Inflammatory Mechanism and YAP/TAZ Factor.Drug Des Devel Ther.2021 Feb 16;15:589-600.doi:10. 2147/DDDT.S294009.PMID:33623368;PMCID
Species: Rat
M00102-2 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Distribution change of mast cells in human nasal polyps

Serum interleukin-6 is not linked with sleep-quality, restless legs syndrome, and depression, but with 6-month survival in hematological malignancies

Expression of the interleukin 6 system in cortical lesions from patients with tuberous sclerosis complex and focal cortical dysplasia type IIb

Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-?B pathway

Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells

Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity

MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3

Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway

Lu Y, Zhang XS, Zhang ZH, Zhou XM, Gao YY, Liu GJ, Wang H, Wu LY, Li W, Hang CH. J Neuroinflammation. 2018 Mar 19;15(1):87. doi: 10.1186/s12974-018-1118-4. Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarach...

Sun W, Liu Db, Li Ww, Zhang Ll, Long Gx, Wang Jf, Mei Q, Hu Gq. Int J Oncol. 2014 May;44(5):1551-60. Doi: 10.3892/Ijo.2014.2323. Epub 2014 Mar 5. Interleukin-6 Promotes The Migration And Invasion Of Nasopharyngeal Carcinoma Cell Lines And Upregula...

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4 Customer Q&As for Anti-human IL6 Antibody Picoband™ (monoclonal, 7D12)

Question

Our team were content with the WB result of your anti-human IL6 antibody (monoclonal, 7D12). However we have been able to see positive staining in left coronary artery secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

According to literature, left coronary artery does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-11-13

Question

Hi Support: I purchased the antibody against IL-6 with the SKU M00102-2 and used it as primary antibody in Western Blot. However, my results obtained 8 colored bands on the membrane (24 to 150kD), in which only one of them corresponds to the right molecular weight (about 24 kD). I want to figure out whether it is non-specific binding or not. Have you encountered the same profile before? Do you know which site of IL-6 is targeted by this antibody SKU M00102-2?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

The mature protein of IL-6 can be highly N-glycosylated so the actual band is much higher than the expected. The sizes of the bands depend on the complexity of the polysaccharide chain. May I know what protocol you used in Western blot, to see if any alterations/optimizations are necessary?

Boster Scientific Support

Answered: 2019-10-14

Question

We are currently using anti-human IL6 antibody (monoclonal, 7D12) M00102-2 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

The anti-human IL6 antibody (monoclonal, 7D12) (M00102-2) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-27

Question

We have observed staining in human left coronary artery. Do you have any suggestions? Is anti-human IL6 antibody (monoclonal, 7D12) supposed to stain left coronary artery positively?

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

From what I have seen in literature left coronary artery does express IL6. From what I have seen in Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-05-24

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M00102-2
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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