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Product Info Summary
| SKU: | PA1586 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TREM1 Antibody Picoband®
SKU/Catalog Number
PA1586
BA4629 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TREM1 Antibody catalog # PA1586. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TREM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1586)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle of mouse TREM1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1586 is reactive to Trem1 in Mouse, Rat
Observed Molecular Weight
22-26 kDa
Calculated molecular weight
25.4 kDa
Background of Trem1
Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1586 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse
Positive Control
WB: rat liver tissue, mouse liver tissue, mouse spleen tissue
IHC: Mouse Spleen Tissue
Validation Images & Assay Conditions
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Anti-TREM1 antibody, PA1586, IHC(P)
IHC(P): Mouse Spleen Tissue
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Anti-TREM1 antibody, PA1586, Western blotting
Lane 1: Recombinant Mouse TREM1 Protein 1ng
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HMGN1, TLR4, and TREM-1 protein levels in the kidney of different experimental groups. (A) Immunohistochemical stain of HMGN1, TLR4 and TREM-1 in the kidney tissue of UUO rats ( n = 5). Original magnification = 400 ×. Scale bars. 50 um. (B) Quantification of the levels of HMGN1, TLR4 and TREM-1 proteins. all values are presented as mean ± SEM. ∗∗ p < 0.001, ∗∗∗ p < 0.0001. Download full-size image DOI:
Index in PubMed under a CC BY license. PMID: 36691481
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Correlation analysis of HMGN1 protein with the key proteins involved in UUO-induced renal inflammation and fibrosis. (A) HMGN1 protein had a positive relation with F4/80+ cells. (B) HMGN1 protein was positively associated with Mcp-1 protein. (C) HMGN1 protein was positively related to KIM-1 protein. (D) HMGN1 protein was positively associated with α -SMA protein. (E) HMGN1 protein was positively associated with β -catenin protein. (F) TLR4 protein and HMGN1 protein had a positive relation. (G) TLR4 protein was positively associated with TREM-1 protein. Download full-size image DOI:
Index in PubMed under a CC BY license. PMID: 36691481
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Western blot analysis of Trem1 using anti-Trem1 antibody (PA1586).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: mouse spleen tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Trem1 antigen affinity purified polyclonal antibody (Catalog # PA1586) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Trem1 at approximately 22-26KD. The expected band size for Trem1 is at 26KD.
Specific Publications For Anti-TREM1 Antibody Picoband® (PA1586)
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1 Customer Q&As for Anti-TREM1 Antibody Picoband®
Question
We are currently using anti-TREM1 antibody PA1586 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on goat tissues as well?
D. Carter
Verified customer
Asked: 2019-07-17
Answer
The anti-TREM1 antibody (PA1586) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-17
