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Product Info Summary
| SKU: | M05470-3 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-Human Kappa Light Chain IGKC Rabbit Monoclonal Antibody, Clone#RM126
SKU/Catalog Number
M05470-3
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human Kappa Light Chain IGKC Rabbit Monoclonal Antibody, Clone#RM126 (Catalog # M05470-3). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Kappa Light Chain IGKC Rabbit Monoclonal Antibody, Clone#RM126 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05470-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM126
Isotype
Rabbit IgG
Immunogen
Human IgG
Cross-reactivity
This antibody reacts to the kappa light chain of human immunoglobulins, and does not react to the lambda light chain. RM126 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M05470-3 is reactive to IGKC in Human
Calculated molecular weight
11.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05470-3 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.05ug/mL – 0.2ug/mL
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL
Flow Cytometry (FC): 0.5ug/mL-2ug/mL.
Validation Images & Assay Conditions
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-human Ig kappa light chain rabbit monoclonal antibody (Clone RM126).
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ELISA result showing specificity
ELISA showing RM126 reacts only to the kappa light chain of human immunoglobulins, and not to lambda light chain, mouse IgG, rat IgG, or goat IgG
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ELISA result showing specificity
A titer ELISA using RM126. The plate was coated with different amounts of human IgG4. A serial dilution of RM126 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
ELISA showing RM126 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM126 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Specific Publications For Anti-Human Kappa Light Chain IGKC Rabbit Monoclonal Antibody, Clone#RM126 (M05470-3)
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