Anti-Toll-like receptor 8 TLR8 Antibody

Boster Bio Anti-Toll-like receptor 8 TLR8 Antibody catalog # RP1073. Tested in WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: RP1073
Size: 100μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Toll-like receptor 8 TLR8 Antibody

See all TLR8 products

SKU/Catalog Number

RP1073

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Toll-like receptor 8 TLR8 Antibody catalog # RP1073. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Toll-like receptor 8 TLR8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1073)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TLR8 (881-907aa DAYISYDTKDASVTDWVINELRYHLEE), identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1073 is reactive to TLR8 in Human, Rat

Applications

RP1073 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For TLR8 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NR97

Gene ID

51311

Gene Name

TLR8

Full Name

Toll-like receptor 8

Weight

119.828kDa

Superfamily

Toll-like receptor family

Alternative Names

CD288 antigen; CD288; MGC119599; MGC119600; TLR8; toll-like receptor 8 TLR8 CD288 toll like receptor 8 toll-like receptor 8

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TLR8, check out the TLR8 Infographic

TLR8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TLR8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Anti-Toll-like receptor 8 TLR8 Antibody

Question

Is this RP1073 anti-TLR8 antibody reactive to the isotypes of TLR8?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

The immunogen of RP1073 anti-TLR8 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TLR8 (881-907aa DAYISYDTKDASVTDWVINELRYHLEE), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-09

Question

I see that the anti-TLR8 antibody RP1073 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-08

Question

Is there a BSA free version of anti-TLR8 antibody RP1073 available?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TLR8 antibody RP1073 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-23

Question

Would anti-TLR8 antibody RP1073 work for WB with placenta?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

According to the expression profile of placenta, TLR8 is highly expressed in placenta. So, it is likely that anti-TLR8 antibody RP1073 will work for WB with placenta.

Boster Scientific Support

Answered: 2020-03-17

Question

Does RP1073 anti-TLR8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

You can see on the product datasheet, RP1073 anti-TLR8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-24

Question

Please see the WB image, lot number and protocol we used for placenta using anti-TLR8 antibody RP1073. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-20

Question

We are currently using anti-TLR8 antibody RP1073 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

The anti-TLR8 antibody (RP1073) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-09

Question

I am interested in using your anti-TLR8 antibody for cellular response to mechanical stimulus studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

I appreciate your inquiry. This RP1073 anti-TLR8 antibody is tested on rat liver tissue, tissue lysate, hepg2 whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-25

Question

Would anti-TLR8 antibody RP1073 work on pig WB with blood?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

Our lab technicians have not tested anti-TLR8 antibody RP1073 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TLR8 antibody RP1073 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-24

Question

I was wanting to use your anti-TLR8 antibody for WB for rat placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat placenta identification?

T. Evans

Verified customer

Asked: 2018-01-08

Answer

As indicated on the product datasheet, RP1073 anti-TLR8 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-08

Question

We have seen staining in human liver. Are there any suggestions? Is anti-TLR8 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2017-12-18

Answer

From what I have seen in literature liver does express TLR8. From what I have seen in Uniprot.org, TLR8 is expressed in blood, placenta, liver, among other tissues. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425

Boster Scientific Support

Answered: 2017-12-18

Question

Is a blocking peptide available for product anti-TLR8 antibody (RP1073)?

Verified Customer

Verified customer

Asked: 2017-08-23

Answer

We do provide the blocking peptide for product anti-TLR8 antibody (RP1073). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-08-23

Question

We need to test anti-TLR8 antibody RP1073 on rat placenta for research purposes, then I may be interested in using anti-TLR8 antibody RP1073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-12

Answer

The products we sell, including anti-TLR8 antibody RP1073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-TLR8 antibody RP1073. Let me know if you need anything else.

J. Evans

Verified customer

Asked: 2015-02-26

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-02-26

Question

Our team were happy with the WB result of your anti-TLR8 antibody. However we have been able to see positive staining in liver membrane using this antibody. Is that expected? Could you tell me where is TLR8 supposed to be expressed?

K. Taylor

Verified customer

Asked: 2014-04-30

Answer

From what I have seen in literature, liver does express TLR8. Generally TLR8 expresses in membrane. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425

Boster Scientific Support

Answered: 2014-04-30

Question

Can you help my question with product RP1073, anti-TLR8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

G. Wu

Verified customer

Asked: 2013-11-08

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1073 anti-TLR8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-11-08

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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RP1073
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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