Product Info Summary
SKU: | DZ33984 |
---|---|
Size: | 200 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC-P, WB |
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Product info
Product Name
Anti-human peroxidasin homolog isoform X1 Antibody
SKU/Catalog Number
DZ33984
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-peroxidasin homolog isoform X1 Antibody catalog # DZ33984. Tested in IHC-P, WB applications. This antibody reacts with human, mouse, rat.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-human peroxidasin homolog isoform X1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ33984)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ33984 is reactive to PXDN in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
165.275kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
DZ33984 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5 μg/ml, Hu, Ms, Rat
Immunohistochemistry(Paraffin-embedded Section), 1 μg/ml, Hu
Positive Control
WB: human Hela whole cell, human U251 whole cell, rat heart tissue, mouse heart tissue
IHC: human pancrease cancer tissue, human renal cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Peroxidasin homolog isoform X1 using anti-Peroxidasin homolog isoform X1 antibody (DZ33984).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: rat heart tissuel lysates,
Lane 4: mouse heart tissuel lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxidasin homolog isoform X1 antigen affinity purified polyclonal antibody (Catalog # DZ33984) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxidasin homolog isoform X1 at approximately 165 kDa.
Click image to see more details
Figure 2. IHC analysis of Peroxidasin homolog isoform X1 using anti-Peroxidasin homolog isoform X1 antibody (DZ33984).
Peroxidasin homolog isoform X1 was detected in paraffin-embedded section of human pancrease cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peroxidasin homolog isoform X1 Antibody (DZ33984) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Peroxidasin homolog isoform X1 using anti-Peroxidasin homolog isoform X1 antibody (DZ33984).
Peroxidasin homolog isoform X1 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peroxidasin homolog isoform X1 Antibody (DZ33984) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Peroxidasin homolog isoform X1 using anti-Peroxidasin homolog isoform X1 antibody (DZ33984).
Peroxidasin homolog isoform X1 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peroxidasin homolog isoform X1 Antibody (DZ33984) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PXDN (Source: Uniprot.org, NCBI)
Gene Name
PXDN
Full Name
Peroxidasin homolog
Weight
165.275kDa
Superfamily
peroxidase family
Alternative Names
peroxidasin homolog (Drosophila) PXDN ASGD7, COPOA, D2S448, D2S448E, MG50, PRG2, PXN, VPO peroxidasin peroxidasin homolog|melanoma-associated MG50|p53-responsive gene 2 protein|vascular peroxidase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PXDN, check out the PXDN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PXDN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human peroxidasin homolog isoform X1 Antibody (DZ33984)
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