Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody
Rabbit IgG polyclonal antibody for Peroxisomal multifunctional enzyme type 2(HSD17B4) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 1 publication(s).
Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody
See all HSD17B4 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for HSD17B4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. HSD17B4 information: Molecular Weight: 79686 MW; Subcellular Localization: Peroxisome; Tissue Specificity: Present in many tissues with highest concentrations in liver, heart, prostate and testis.|
|Cite This Product||Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1727)|
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|Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody may replace the following items: sc 131141|sc 131142|sc 135045|sc 271825|sc 365167|sc 390146|sc 66423|sc 81073|sc 98411.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Hydroxysteroid(17-beta) Dehydrogenase 4(744-761aa NIMLSQKLQMILKDYAKL), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Peroxisomal multifunctional enzyme type 2|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids. .|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Our Boster Quality Guarantee for Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: human placenta tissue lysates,
Lane 4: MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- HSD17B4 antigen affinity purified polyclonal antibody (Catalog # PA1727) at 0.5 Î¼g/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B4 at approximately 80KD. The expected band size for HSD17B4 is at 80KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
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- Flow Cytometry Technical Resource Center
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