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- Table of Contents
AIF1 antibody (M01394-4) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of IBA1/AIF1 using anti-IBA1/AIF1 antibody (M01394-4).
IBA1/AIF1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IBA1/AIF1 Antibody (M01394-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
IBA1/AIF1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IBA1/AIF1 Antibody (M01394-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Iba1 Rabbit Monoclonal Antibody
AIF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
AIF1 antibody was positively detected by WB in:
human HEL whole cell, human THP-1 whole cell, human K562 whole cell, rat liver tissue, rat spleen tissue, mouse liver tissue, mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AIF1 antibody was positively detected by IHC in:
human spleen tissue, human tonsil tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Iba1 Rabbit Monoclonal Antibody
View all AIF1 Antibodies
View all AIF1 Antibodies
Form
Liquid
Uniprot
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31A74
Isotype
IgG
Immunogen
A synthesized peptide derived from human Iba1
Observed Molecular Weight
17 kDa
Cite This Product
Anti-Iba1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01394-4)
Protocols
Western Blot protocol for AIF1 antibody
Immunohistochemistry (IHC) protocol for AIF1 antibody
Publications
Application:
Species:
The novel GLP-1/GIP dual receptor agonist DA5-CH is superior to tirzepatide and exendin-4 in the 6-OHDA Parkinson rat model
Published: 2026-04-17
Journal: Frontiers in Endocrinology
Application: IHC
Impact Factor: 4.60
Species: Rat
PMID: 42165019
Hamayou (Oviductus Ranae) protein hydrolysate ameliorates depression by regulating the mitogen-activated protein kinase pathway
Published: 2025-05-21
Journal: Journal of Traditional Chinese Medicine
Application: IF
Impact Factor: 2.20
Species: Mouse
PMID: 40524289
Review
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1 Review For Anti-Iba1 Rabbit Monoclonal Antibody
In the IF experiment using Anti-IBA-1 antibody (Cat# M01394-4), the antibody clearly and specifically labeled microglial cells in mouse cerebral infarction tissue, showing distinct staining and well-defined cellular morphology.
| SKU | M01394-4 |
|---|---|
| Application | Immunofluorescence |
| Sample | mouse brain tissue |
| Sample Processing Description | Mouse cerebral infarction model; brain tissues were collected, fixed in formaldehyde for 48 hours, and then sagittally paraffin-embedded. |
| Other Reagents | Goat serum, DAPI Staining Solution, Antifade fluorescence mounting medium. |
| Primary Antibody | Iba1 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:100, overnight at 4 ℃ |
| Secondary Antibody | Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated (BA1142, Boster) |
| Secondary Incubation | 45 min at 37℃ |
| Detection | Imaging system:Leica DMi3000 |
| Results Summary | IBA-1 is a well-established marker of microglia in the nervous system. Microglia are the primary immune effector cells in the central nervous system and respond rapidly to CNS injury by proliferating, upregulating or re-expressing MHC antigens, migrating, and adopting a phagocyte-like morphology. In this study, IBA-1 was used to label microglia in cerebral infarction samples, showing clear staining and accurate cellular morphology. |
Yu Lei, School of Life Sciences, Wuhan University
Verified customer
Submitted 2026-02-26
Product Promise
We promise Anti-Iba1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01394-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026