Product Info Summary
| SKU: | M03920 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-IFIT3 Mouse Monoclonal Antibody [Clone ID: OTI1G1]
SKU/Catalog Number
M03920
Size
100 µl
Description
Boster Bio Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1). Catalog# M03920. Tested in FC, IF, WB. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-IFIT3 Mouse Monoclonal Antibody [Clone ID: OTI1G1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03920)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1G1
Isotype
IgG1
Immunogen
Full length human recombinant protein of human IFIT3 (NP_001540) produced in HEK293T cell.
Reactive Species
M03920 is reactive to IFIT3 in Human, Monkey
Calculated molecular weight
56.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03920 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (lane 1) , human IFIT3 plasmid (lane 2), mouse IFIT3 plasmid (lane 3) using anti-IFIT3 antibody M03920 (1:1000). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody, 1:1000)
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Western blot analysis of extracts (50ug per lane) from 5 cell lines lysates by using anti-IFIT3 monoclonal antibody(M03920, 1:1000)
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Western blot analysis of extracts (30ug per lane) from 4 cell lines lysates by using anti-IFIT3 monoclonal antibody(M03920, 1:500)
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Anti-IFIT3 mouse monoclonal antibody (M03920) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT3.
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Immunofluorescent staining of HT29 cells using anti-IFIT3 mouse monoclonal antibody (M03920).
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Flow cytometric analysis of Jurkat cells, using antiIFIT3 antibody (M03920), (Red) compared to a nonspecific negative control antibody (Blue).
Specific Publications For Anti-IFIT3 Mouse Monoclonal Antibody [Clone ID: OTI1G1] (M03920)
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