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Product Info Summary
| SKU: | PA2183 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IRAK/IRAK1 Antibody Picoband®
SKU/Catalog Number
PA2183
BA3462-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IRAK/IRAK1 Antibody catalog # PA2183. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRAK/IRAK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2183)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IRAK, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2183 is reactive to IRAK1 in Human, Mouse, Rat
Observed Molecular Weight
274 kDa
Calculated molecular weight
76.5 kDa
Background of IRAK1
Interleukin-1 receptor-associated kinase 1, also called IRAK1, is an enzyme that in humans is encoded by the IRAK1 gene. By radiation hybrid analysis, this gene is mapped to chromosome Xq28. Serine/threonine-protein kinase plays a critical role in initiating innate immune response against foreign pathogens. This gene involved in Toll-like receptor (TLR) and IL-1R signaling pathways. This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2183 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Liver Tissue, Human Placenta Tissue, MCF-7 Cell, PANC Cell,
Validation Images & Assay Conditions
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Anti-IRAK antibody, PA2183, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: PANC Cell Lysate
Specific Publications For Anti-IRAK/IRAK1 Antibody Picoband® (PA2183)
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Customer Q&As
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4 Customer Q&As for Anti-IRAK/IRAK1 Antibody Picoband®
Question
We are currently using anti-IRAK/IRAK1 antibody PA2183 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-IRAK/IRAK1 antibody (PA2183) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
Would anti-IRAK/IRAK1 antibody PA2183 work on dog WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Our lab technicians have not tested anti-IRAK/IRAK1 antibody PA2183 on dog. You can run a BLAST between dog and the immunogen sequence of anti-IRAK/IRAK1 antibody PA2183 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
My question regarding product PA2183, anti-IRAK/IRAK1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2183 anti-IRAK/IRAK1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-05
Question
Will PA2183 anti-IRAK/IRAK1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Miller
Verified customer
Asked: 2014-04-11
Answer
You can see on the product datasheet, PA2183 anti-IRAK/IRAK1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-04-11
