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- Table of Contents
IFNG antibody (PA1493) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Anti-IFN gamma antibody, PA1493, Western blotting
All lanes: Anti IFN gamma (PA1493) at 0.5ug/ml
Lane 1: Recombinant Human IFN gamma Protein 10ng
Lane 2: Recombinant Human IFN gamma Protein 5ng
Lane 3: Recombinant Human IFN gamma Protein 2.5ng
Lane 4: Recombinant Human IFN gamma Protein 1.25ng
Predicted bind size: 19KD
Observed bind size: 19KD
All lanes: Anti IFN gamma (PA1493) at 0.5ug/ml
Lane 1: Recombinant Human IFN gamma Protein 10ng
Lane 2: Recombinant Human IFN gamma Protein 5ng
Lane 3: Recombinant Human IFN gamma Protein 2.5ng
Lane 4: Recombinant Human IFN gamma Protein 1.25ng
Predicted bind size: 19KD
Observed bind size: 19KD
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Anti-Interferon gamma/IFNG Antibody Picoband®
IFNG antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
IFNG antibody was positively detected by WB in:
recombinant human IFN gamma protein
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Interferon gamma/IFNG Antibody Picoband®
View all IFNG Antibodies
View all IFNG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IFN gamma.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated Molecular Weight
19.3 kDa
Background of IFNG
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Cite This Product
Anti-Interferon gamma/IFNG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1493)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Rabbit
88%
Moderate Homology
Cow
88%
Moderate Homology
Sheep
88%
Moderate Homology
Dog
88%
Moderate Homology
Mouse
31%
Low homology
Rat
31%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for IFNG antibody
Publications
Application:
Species:
The peptide-based bispecific CAR T cells target EGFR and tumor stroma for effective cancer therapy
Published: 2024-08-05
Journal: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Application: IHC
Impact Factor: 5.30
Species: Mouse
PMID: 39111352
Peptide Blocking of PD-1/PD-L1 Interaction for Cancer Immunotherapy
Published: 2018-01-31
Journal: Cancer Immunology Research
Application: IHC
Impact Factor: 9.19
Species: Mouse
PMID: 29217732
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Product Promise
We promise Anti-Interferon gamma/IFNG Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA1493
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026