Product Info Summary
| SKU: | PA1493 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interferon gamma/IFNG Antibody Picoband®
SKU/Catalog Number
PA1493
BA0952 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interferon gamma/IFNG Antibody catalog # PA1493. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon gamma/IFNG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1493)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IFN gamma.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1493 is reactive to IFNG in Human
Observed Molecular Weight
19 kDa
Calculated molecular weight
19.3 kDa
Background of IFNG
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1493 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-IFN gamma antibody, PA1493, Western blotting
All lanes: Anti IFN gamma (PA1493) at 0.5ug/ml
Lane 1: Recombinant Human IFN gamma Protein 10ng
Lane 2: Recombinant Human IFN gamma Protein 5ng
Lane 3: Recombinant Human IFN gamma Protein 2.5ng
Lane 4: Recombinant Human IFN gamma Protein 1.25ng
Predicted bind size: 19KD
Observed bind size: 19KD
Specific Publications For Anti-Interferon gamma/IFNG Antibody Picoband® (PA1493)
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Customer Q&As
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3 Customer Q&As for Anti-Interferon gamma/IFNG Antibody Picoband®
Question
We are currently using anti-Interferon gamma/IFNG antibody PA1493 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
The anti-Interferon gamma/IFNG antibody (PA1493) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-06
Question
We have been able to see staining in human blood. What should we do? Is anti-Interferon gamma/IFNG antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
From literature blood does express IFNG. From Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-10-13
Question
We were content with the WB result of your anti-Interferon gamma/IFNG antibody. However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
From what I have seen in literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-09-18


