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Product Info Summary
| SKU: | PB9024 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Interferon gamma/IFNG Antibody Picoband®
SKU/Catalog Number
PB9024
PB0062 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interferon gamma/IFNG Antibody Picoband® catalog # PB9024. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon gamma/IFNG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9024)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat IFN gamma recombinant protein (Position: Q23-C156). Rat IFN gamma shares 38% and 86% amino acid (aa) sequences identity with human and mouse IFN gamma, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9024 is reactive to Ifng in Rat
Observed Molecular Weight
19 kDa
Calculated molecular weight
17.9 kDa
Background of Ifng
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9024 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Specific Publications For Anti-Interferon gamma/IFNG Antibody Picoband® (PB9024)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Interferon gamma/IFNG Antibody Picoband®
Question
Is this PB9024 anti-Interferon gamma/IFNG antibody reactive to the isotypes of IFNG?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The immunogen of PB9024 anti-Interferon gamma/IFNG antibody is E.coli-derived rat IFN gamma recombinant protein (Position: Q23-C156). Rat IFN gamma shares 38% and 86% amino acid (aa) sequences identity with human and mouse IFN gamma, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-02
Question
We have been able to see staining in rat blood. Do you have any suggestions? Is anti-Interferon gamma/IFNG antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
From what I have seen in literature blood does express IFNG. From what I have seen in Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-13
Question
See attached the WB image, lot number and protocol we used for leukocyte using anti-Interferon gamma/IFNG antibody PB9024. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
We are interested in to test anti-Interferon gamma/IFNG antibody PB9024 on rat leukocyte for research purposes, then I may be interested in using anti-Interferon gamma/IFNG antibody PB9024 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
H. Collins
Verified customer
Asked: 2019-08-19
Answer
The products we sell, including anti-Interferon gamma/IFNG antibody PB9024, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-19
Question
Will PB9024 anti-Interferon gamma/IFNG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
As indicated on the product datasheet, PB9024 anti-Interferon gamma/IFNG antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-05
Question
My colleagues were well pleased with the WB result of your anti-Interferon gamma/IFNG antibody. However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?
S. Anderson
Verified customer
Asked: 2018-11-26
Answer
According to literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-26
Question
Will anti-Interferon gamma/IFNG antibody PB9024 work for WB with leukocyte?
D. Williams
Verified customer
Asked: 2018-11-14
Answer
According to the expression profile of leukocyte, IFNG is highly expressed in leukocyte. So, it is likely that anti-Interferon gamma/IFNG antibody PB9024 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2018-11-14
Question
We are currently using anti-Interferon gamma/IFNG antibody PB9024 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says rat. Is it true that the antibody can work on zebrafish tissues as well?
V. Li
Verified customer
Asked: 2018-10-18
Answer
The anti-Interferon gamma/IFNG antibody (PB9024) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-18
Question
I was wanting to use your anti-Interferon gamma/IFNG antibody for WB for rat leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukocyte identification?
P. Johnson
Verified customer
Asked: 2018-05-11
Answer
You can see on the product datasheet, PB9024 anti-Interferon gamma/IFNG antibody has been tested for ELISA, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-11
Question
Our lab used your anti-Interferon gamma/IFNG antibody for WB on leukocyte a few years ago. I am using rat, and We are going to use the antibody for ELISA next. I was wanting to use examining leukocyte as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
R. Krishna
Verified customer
Asked: 2017-12-26
Answer
I have checked the website and datasheets of our anti-Interferon gamma/IFNG antibody and it appears that PB9024 has been validated on rat in both WB and ELISA. Thus PB9024 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-26
Question
Is there a BSA free version of anti-Interferon gamma/IFNG antibody PB9024 available?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Interferon gamma/IFNG antibody PB9024 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-17
Question
My question regarding product PB9024, anti-Interferon gamma/IFNG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Miller
Verified customer
Asked: 2017-03-14
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9024 anti-Interferon gamma/IFNG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-14
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Interferon gamma/IFNG antibody PB9024. Let me know if you need anything else.
M. Yang
Verified customer
Asked: 2016-04-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-04-08
Question
I see that the anti-Interferon gamma/IFNG antibody PB9024 works with WB, what is the protocol used to produce the result images on the product page?
J. Huang
Verified customer
Asked: 2016-03-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-03-23
Question
Is a blocking peptide available for product anti-Interferon gamma/IFNG antibody (PB9024)?
W. Taylor
Verified customer
Asked: 2014-01-24
Answer
We do provide the blocking peptide for product anti-Interferon gamma/IFNG antibody (PB9024). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-01-24
