Anti-IKK alpha/CHUK Antibody

CHUK antibody

Boster Bio Anti-IKK alpha/CHUK Antibody catalog # PA1614. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1614
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-IKK alpha/CHUK Antibody

View all CHUK Antibodies

SKU/Catalog Number

PA1614

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IKK alpha/CHUK Antibody catalog # PA1614. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IKK alpha/CHUK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1614)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human IKK alpha.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1614 is reactive to CHUK in Human

Applications

PA1614 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

84.64kDa

Background of CHUK

IKBKA (INHIBITOR OF KAPPA LIGHT POLYPEPTIDE GENE ENHANCER IN B CELLS, KINASE OF, ALPHA), also called CHUK, NFKBIKA, IKKA, IKK1, I-KAPPA-B, is a protein kinase that in humans is encoded by the CHUK gene. And it is a member of the protein kinase superfamily. And the protein contains a serine/threonine kinase domain, due to its ubiquitous expression in a broad array of tissues and high degree of conservation across species. By FISH, the IKBKA gene is mapped to chromosome 10q24. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. IKK-alpha is part of the I kappa B kinase complex that plays an important role in regulating the NF-kappa B transcription factor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CHUK (Source: Uniprot.org, NCBI)

Gene Name

CHUK

Full Name

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Weight

84.64kDa

Superfamily

protein kinase superfamily

Alternative Names

CHUK; conserved helix-loop-helix ubiquitous kinaseIKBKA; EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkBKA; IkBKalpha; IKK alpha; IKK1; IKK1I-kappa-B kinase-alpha; IKK-A; IKKAIkB kinase alpha subunit; IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; NFKBIKAIKK-a kinase; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; TCF16; TCF-16; Transcription factor 16 CHUK IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 component of inhibitor of nuclear factor kappa B kinase complex inhibitor of nuclear factor kappa-B kinase subunit alpha|I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|TCF-16|conserved helix-loop-helix ubiquitous kinase|transcription factor 16

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CHUK, check out the CHUK Infographic

CHUK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CHUK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-IKK alpha/CHUK Antibody

Question

Does anti-IKK alpha/CHUK antibody PA1614 work for WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

According to the expression profile of cervix carcinoma, CHUK is highly expressed in cervix carcinoma. So, it is likely that anti-IKK alpha/CHUK antibody PA1614 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2019-12-02

Question

I see that the anti-IKK alpha/CHUK antibody PA1614 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-13

Question

I was wanting to use your anti-IKK alpha/CHUK antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

You can see on the product datasheet, PA1614 anti-IKK alpha/CHUK antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-24

Question

Do you have a BSA free version of anti-IKK alpha/CHUK antibody PA1614 available?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IKK alpha/CHUK antibody PA1614 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-23

Question

Is a blocking peptide available for product anti-IKK alpha/CHUK antibody (PA1614)?

Verified Customer

Verified customer

Asked: 2017-11-30

Answer

We do provide the blocking peptide for product anti-IKK alpha/CHUK antibody (PA1614). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-30

Size

Conjugation

Total: $370

SKU:PA1614

In stock, 1 left.

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PA1614
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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