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Product Info Summary
| SKU: | PA1614 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-IKK alpha/CHUK Antibody Picoband®
SKU/Catalog Number
PA1614
BA1594-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IKK alpha/CHUK Antibody catalog # PA1614. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKK alpha/CHUK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1614)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IKK alpha.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1614 is reactive to CHUK in Human
Observed Molecular Weight
85 kDa
Calculated molecular weight
84.6 kDa
Background of CHUK
IKBKA (INHIBITOR OF KAPPA LIGHT POLYPEPTIDE GENE ENHANCER IN B CELLS, KINASE OF, ALPHA), also called CHUK, NFKBIKA, IKKA, IKK1, I-KAPPA-B, is a protein kinase that in humans is encoded by the CHUK gene. And it is a member of the protein kinase superfamily. And the protein contains a serine/threonine kinase domain, due to its ubiquitous expression in a broad array of tissues and high degree of conservation across species. By FISH, the IKBKA gene is mapped to chromosome 10q24. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. IKK-alpha is part of the I kappa B kinase complex that plays an important role in regulating the NF-kappa B transcription factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1614 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Raji whole cell, human HepG2 whole cell, human Hela whole cell
FCM: Raji cell
Validation Images & Assay Conditions
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Western blot analysis of IKK Alpha/CHUK using anti-IKK Alpha/CHUK antibody (PA1614).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK Alpha/CHUK antigen affinity purified polyclonal antibody (Catalog # PA1614) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK Alpha/CHUK at approximately 85 kDa. The expected band size for IKK Alpha/CHUK is at 85 kDa.
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Flow Cytometry analysis of Raji cells using anti-IKK Alpha/CHUK antibody (PA1614).
Overlay histogram showing Raji cells stained with PA1614 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK Alpha/CHUK Antibody (PA1614, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-IKK alpha/CHUK Antibody Picoband® (PA1614)
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Customer Q&As
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5 Customer Q&As for Anti-IKK alpha/CHUK Antibody Picoband®
Question
Does anti-IKK alpha/CHUK antibody PA1614 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
According to the expression profile of cervix carcinoma, CHUK is highly expressed in cervix carcinoma. So, it is likely that anti-IKK alpha/CHUK antibody PA1614 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-12-02
Question
I see that the anti-IKK alpha/CHUK antibody PA1614 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-13
Question
I was wanting to use your anti-IKK alpha/CHUK antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
You can see on the product datasheet, PA1614 anti-IKK alpha/CHUK antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-24
Question
Do you have a BSA free version of anti-IKK alpha/CHUK antibody PA1614 available?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IKK alpha/CHUK antibody PA1614 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-23
Question
Is a blocking peptide available for product anti-IKK alpha/CHUK antibody (PA1614)?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
We do provide the blocking peptide for product anti-IKK alpha/CHUK antibody (PA1614). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-11-30
