Product Info Summary
SKU: | PA1614 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-IKK alpha/CHUK Antibody
SKU/Catalog Number
PA1614
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IKK alpha/CHUK Antibody catalog # PA1614. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKK alpha/CHUK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1614)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IKK alpha.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1614 is reactive to CHUK in Human
Applications
PA1614 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
84.64kDa
Background of CHUK
IKBKA (INHIBITOR OF KAPPA LIGHT POLYPEPTIDE GENE ENHANCER IN B CELLS, KINASE OF, ALPHA), also called CHUK, NFKBIKA, IKKA, IKK1, I-KAPPA-B, is a protein kinase that in humans is encoded by the CHUK gene. And it is a member of the protein kinase superfamily. And the protein contains a serine/threonine kinase domain, due to its ubiquitous expression in a broad array of tissues and high degree of conservation across species. By FISH, the IKBKA gene is mapped to chromosome 10q24. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. IKK-alpha is part of the I kappa B kinase complex that plays an important role in regulating the NF-kappa B transcription factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-IKK alpha antibody, PA1614, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For CHUK (Source: Uniprot.org, NCBI)
Gene Name
CHUK
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Weight
84.64kDa
Superfamily
protein kinase superfamily
Alternative Names
CHUK; conserved helix-loop-helix ubiquitous kinaseIKBKA; EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkBKA; IkBKalpha; IKK alpha; IKK1; IKK1I-kappa-B kinase-alpha; IKK-A; IKKAIkB kinase alpha subunit; IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; NFKBIKAIKK-a kinase; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; TCF16; TCF-16; Transcription factor 16 CHUK IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 component of inhibitor of nuclear factor kappa B kinase complex inhibitor of nuclear factor kappa-B kinase subunit alpha|I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|TCF-16|conserved helix-loop-helix ubiquitous kinase|transcription factor 16
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHUK, check out the CHUK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHUK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKK alpha/CHUK Antibody (PA1614)
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No publications found for PA1614
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-IKK alpha/CHUK Antibody
Question
Does anti-IKK alpha/CHUK antibody PA1614 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
According to the expression profile of cervix carcinoma, CHUK is highly expressed in cervix carcinoma. So, it is likely that anti-IKK alpha/CHUK antibody PA1614 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-12-02
Question
I see that the anti-IKK alpha/CHUK antibody PA1614 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-13
Question
I was wanting to use your anti-IKK alpha/CHUK antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
You can see on the product datasheet, PA1614 anti-IKK alpha/CHUK antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-24
Question
Do you have a BSA free version of anti-IKK alpha/CHUK antibody PA1614 available?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IKK alpha/CHUK antibody PA1614 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-23
Question
Is a blocking peptide available for product anti-IKK alpha/CHUK antibody (PA1614)?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
We do provide the blocking peptide for product anti-IKK alpha/CHUK antibody (PA1614). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-30