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Product Info Summary
| SKU: | A01918S176 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IKK Alpha / Beta (phospho-S176/177) CHUK Antibody
SKU/Catalog Number
A01918S176
Size
100ul
Form
Liquid
Description
Boster Bio Anti-IKK Alpha / Beta (phospho-S176/177) CHUK Antibody catalog # A01918S176. Tested in WB,IHC applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKK Alpha / Beta (phospho-S176/177) CHUK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01918S176)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human IKKα/β around the phosphorylation site of Serine 176/177.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01918S176 is reactive to CHUK in Human, Mouse
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01918S176 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of IKKα/β (phospho-S176/177) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with TNFα(20ng/ml)for 10 minutes whole cell lysate Lane3:HEK293T treated with TNFα(20ng/ml)for 40 minutes whole cell lysate Lane4:HEK293T treated with TNFα(20ng/ml)for 1 hour whole cell lysate
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Immunohistochemistry (IHC) analyzes of p-IKKα/β (S176/177) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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Immunohistochemistry (IHC) analyzes of p-IKKα/β (S176/177) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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Immunohistochemistry (IHC) analyzes of p-IKKα/β (S176/177) pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Specific Publications For Anti-IKK Alpha / Beta (phospho-S176/177) CHUK Antibody (A01918S176)
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