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Product Info Summary
| SKU: | A05256 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL-31R alpha Antibody
SKU/Catalog Number
A05256
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IL-31R alpha Antibody catalog # A05256. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-31R alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05256)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human IL-31Rα.
Reactive Species
A05256 is reactive to IL31RA in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
83.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05256 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of extracts from Jurkat cells, using IL-31R Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-IL-31R alpha Antibody (A05256)
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Customer Q&As
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7 Customer Q&As for Anti-IL-31R alpha Antibody
Question
Will anti-IL-31R alpha antibody A05256 work on zebrafish WB with thoracic aorta?
R. Dhar
Verified customer
Asked: 2020-02-03
Answer
Our lab technicians have not tested anti-IL-31R alpha antibody A05256 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IL-31R alpha antibody A05256 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish thoracic aorta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-03
Question
We are currently using anti-IL-31R alpha antibody A05256 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The anti-IL-31R alpha antibody (A05256) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-02
Question
I have a question about product A05256, anti-IL-31R alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05256 anti-IL-31R alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thoracic aorta using anti-IL-31R alpha antibody A05256. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-24
Question
Is a blocking peptide available for product anti-IL-31R alpha antibody (A05256)?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
We do provide the blocking peptide for product anti-IL-31R alpha antibody (A05256). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-02
Question
I see that the anti-IL-31R alpha antibody A05256 works with WB, what is the protocol used to produce the result images on the product page?
A. Jones
Verified customer
Asked: 2016-06-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-06-16
Question
Please see the WB image, lot number and protocol we used for thoracic aorta using anti-IL-31R alpha antibody A05256. Please let me know if you require anything else.
C. Carter
Verified customer
Asked: 2016-02-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-18
