Human IL-31/Interleukin-31 ELISA Kit PicoKine™

Human IL-31 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL31 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0979
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human IL-31/Interleukin-31 ELISA Kit PicoKine™

See all IL-31 products

SKU/Catalog Number

EK0979

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-31 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL31 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-31/Interleukin-31 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0979)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-T164

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0979 is reactive to IL31 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0979 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0979-CAP Anti-Human IL31 Pre-coated 96-well strip microplate 1
EK0979-ST Human IL31 Standard 2 vials, 10 ng/tube
EK0979-DA Human IL31 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0040.1420.1970.3160.5860.9841.4462.096

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-31, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-31 in samples: 21294778 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-31/Interleukin-31 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13480419071407401811
Standard deviation8.0444.22122.0410.552.54130.39
CV (%)6%5.5%6.4%7.5%7.1%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-31 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11341221291161256.835.4%
Sample 280476582774178433.384.2%
Sample 319071624162318031739121.456.9%
*number of samples for each test n=16.

Gene/Protein Information For IL31 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q6EBC2

Gene ID

386653

Gene Name

IL31

Full Name

Interleukin-31

Weight

18.205kDa

Alternative Names

IL31; IL-31; IL-31interleukin-31; interleukin 31 IL31 IL-31 interleukin 31 interleukin-31

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL31, check out the IL31 Infographic

IL31 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL31: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0979 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Haggag MM,Kora MA,Safan MA,Yasien HA,Yousef AM.Study of serum level of interleukin-31 in patients with uremic pruritus.Menoufia Med J 2020;33:257-61
Species: Human
EK0979 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

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5 Customer Q&As for Human IL-31/Interleukin-31 ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Human IL-31 Picokine® ELISA Kit (Catalog # EK0979)?

Z. Collins

Verified customer

Asked: 2020-08-24

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-31 causing disruption of its protein structure. IL-31 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-31 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-08-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

C. Brown

Verified customer

Asked: 2020-07-22

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-07-22

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

L. Li

Verified customer

Asked: 2018-04-23

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-04-23

Question

Q: What is the optimal O.D. value for IL-31 ELISA kit? I used your IL-31 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-31 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2016-07-15

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-31 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-31 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2016-07-15

Question

Q: if the enzyme conjugated IL-31 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-31 antigen?

R. Wu

Verified customer

Asked: 2014-04-11

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-04-11

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