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Product Info Summary
| SKU: | M02925 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti- IL-9 Monoclonal Antibody
SKU/Catalog Number
M02925
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- IL-9 Monoclonal Antibody catalog # M02925. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IL-9 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02925)
Host
Mouse
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFID-9
Isotype
Mouse IgG2b, κ
Immunogen
Human IL-9 coupled to ovalbumin
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02925 is reactive to IL9 in Mouse, Rat
Observed Molecular Weight
16 kDa
Calculated molecular weight
15.9 kDa
Background of IL9
The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02925 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5&mu:g/ml, Mouse, Rat
Positive Control
WB: rat spleen tissue, rat thymus tissue, mouse thymus tissue, mouse RAW2647 whole cell
Validation Images & Assay Conditions
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Western blot analysis of IL-9 using anti-IL-9 antibody (M02925).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse thymus tissue lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IL-9 antigen affinity purified monoclonal antibody (Catalog # M02925) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IL-9 at approximately 16KD. The expected band size for IL-9 is at 16KD.
Specific Publications For Anti- IL-9 Monoclonal Antibody (M02925)
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