Rabbit IgG polyclonal antibody for Interleukin-3 receptor subunit alpha(IL3RA) detection. Tested with WB in Human.
Anti-IL3RA Antibody Info At A Glance
Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
|Product Name||Anti-IL3RA Antibody
See all IL-3R alpha/CD123 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CD123/IL3RA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. CD123/IL3RA information: Molecular Weight: 43330 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein.|
|Cite This Product||Anti-IL3RA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1617)|
|Similar Products From
|Anti-IL3RA Antibody may replace the following items: sc 30006|sc 455|sc 681|sc 80650|sc 80651|sc 83881|sc 83882.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human IL3RA(135-151aa QYDLYLNVANRRQQYEC).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For IL3RA (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3563|
|Species Of This Entry||Human|
|Protein Name||Interleukin-3 receptor subunit alpha|
|Superfamily||type I cytokine receptor family|
|Alternative Names||IL-3R alpha/CD123|CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174|
|Post Tranlational Modifications||Phosphorylation; Methylation; Acetylation|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Gene Location||Xp22.33 and Yp11.2, on Chromasome X|Y, gene sequence: NC_000023.11 (1336574..1382689)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL3RA (Source: Uniprot.org, NCBI)
|Protein Function||This is a receptor for interleukin-3.|
|Research Areas||Cytokine Research; Phospho-Specific|
|Related Diseases||Leukemia; Neoplasms; Myeloid Leukemia; Leukemia, Myelocytic, Acute; Lymphoma; Malignant Neoplasms; Skin Neoplasms; Hiv Infections; Malignant Paraganglionic Neoplasm; Immunologic Deficiency Syndromes; Hematologic Neoplasms; Lymphoid Leukemia; Acute Leukemia; Carcinoma; Lymphoma, Non-hodgkin; Infective Disorder; Sclerosis; Dermatologic Disorders; Chronic Lymphocytic Leukemia|
|Related Pathways||Immune Response; Pathogenesis; Secretion; Cell Differentiation; Cell Proliferation; Cell Cycle; Endocytosis; Localization; Cytokine Production; Inflammatory Response; Innate Immune Response; Regeneration; Cell Growth; Dendritic Cell Proliferation; Cell Adhesion; T Cell Selection; Transposition; Cell Migration; Osteoblast Differentiation; Cytokine Secretion|
|Background||IL3RA(INTERLEUKIN 3 RECEPTOR, ALPHA), also called CD123(Cluster of Differentiation 123), is a human gene. The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor which is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3(IL3), colony stimulating factor 2(CSF2/GM-CSF), and interleukin 5(IL5). The IL3RA is mapped on Xp22.33. The genomic structures of IL3RA and CSF2RA are very similar and share an additional exon encoding part of the C-terminal domain not found in other members of this gene family. As in human hematopoietic cells, IL3 and GMCSF competed for binding in fibroblasts expressing the cDNAs for IL3RA, CSF2RA, and the common beta subunit, indicating that different alpha subunits compete for a common beta subunit.|
|Scientific References||PMID: 1833064 by Kitamura T., et al. Expression cloning of the human IL-3 receptor cDNA reveals a shared beta subunit for the human IL-3 and GM-CSF receptors.|
PMID: 7887951 by Kosugi H., et al. Structure of the gene encoding the alpha subunit of the human interleukin 3 receptor.
Our Boster Quality Guarantee for Anti-IL3RA Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: A431 Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: 293T Cell Lysate
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