Data & Images
|Product Name||Anti-IL5RA/Cd125 Antibody|
|Description||Rabbit IgG polyclonal antibody for Interleukin-5 receptor subunit alpha(IL5RA) detection. Tested with WB in Human.|
|Cite This Product||Anti-IL5RA/Cd125 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1619)|
|Replacement Item||This antibody may replace the following items: sc-21904|sc-30009|sc-673 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human IL5RA(53-68aa NPDQEQRNVNLEYQVK).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Interleukin-5 receptor subunit alpha(IL-5 receptor subunit alpha/IL-5R subunit alpha/IL-5R-alpha/IL-5RA)|
|Molecular Weight||47685 MW|
|Protein Function||This is the receptor for interleukin-5. The alpha chain binds to IL5.|
|Tissue Specificity||Expressed on eosinophils and basophils.|
|Sequence Similarities||Belongs to the type I cytokine receptor family. Type 5 subfamily.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Interleukin-5 receptor subunit alpha;IL-5 receptor subunit alpha;IL-5R subunit alpha;IL-5R-alpha;IL-5RA;CDw125;CD125;IL5RA;IL5R;|
|Research Areas|||immunology|innate immunity|cytokines|interleukins||
Background for Interleukin-5 receptor subunit alpha(IL-5 receptor subunit alpha/IL-5R subunit alpha/IL-5R-alpha/IL-5RA)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-IL5RA/Cd125 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-IL5RA/Cd125 Antibody Images
Click the images to enlarge.
All lanes: Anti IL5RA (PA1619) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Lane 8: RAJI Whole Cell Lysate at 40ug
Lane 9: CEM Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 60KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,