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Product Info Summary
|Size:||For 5 plates, 96 wells each, reagents only, plates are not included|
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Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
For 5 plates, 96 wells each, reagents only, plates are not included
*Question: How many samples can I assay/run in this kit?
Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL5 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0407)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: sf21; Immunogen sequence: I20-S134
7.8 pg/ml - 500 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EZ0407 is reactive to IL5 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
EZ0407 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EZ0407-CA||Mouse anti- human IL5 monoclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol|
|EZ0407-DA||Biotinylated goat anti- human IL5 polyclonal antibody (Detection Antibody)||500 µl, 2.8 µg/mL (recommended dilution 1:100)||0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol|
|EZ0407-ST||Lyophilized recombinant human IL5 standard||10 ng/tube×2|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. 96 well microplate (Cat# AR1100)
- 6. Plate Sealers.
- 7. Capture Antibody Diluent: PBS.
- 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 11. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
Innovating Scientists Reward
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human IL-5 EZ Set ELISA Kit standard curve
Gene/Protein Information For IL5 (Source: Uniprot.Org, NCBI)
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I IL5 EDF, IL-5, TRF interleukin 5 interleukin-5|B-cell differentiation factor I|T-cell replacing factor|colony-stimulating factor, eosinophil|eosinophil differentiation factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL5, check out the IL5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EZ0407
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