Anti-Integrin alpha 1/ITGA1 Antibody

Rabbit IgG polyclonal antibody for Integrin alpha-1(ITGA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

SKU PA1045-1
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-Integrin alpha 1/ITGA1 Antibody
See all ITGA1 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1045-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Polyclonal antibody for CD49a/ITGA1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CD49a/ITGA1 information: Molecular Weight: 130848 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein.
Cite This Product Anti-Integrin alpha 1/ITGA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1045-1)
Specificity Anti-Integrin alpha 1/ITGA1 Antibody (PA1045-1) reacts with Human, Mouse, Rat ITGA1, in native form and recombinant. Superfamily members of ITGA1 are not reactive to PA1045-1.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 1(1121-1134aa SNQKRELAIQISKD), different from the related mouse and rat sequences by one amino acid.
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-Integrin alpha 1/ITGA1 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Integrin alpha 1/ITGA1 Antibody (PA1045-1).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

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Anti-Integrin alpha 1 antibody, PA1045-1, IHC(P)
IHC(P): Human Lung Cancer Tissue

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Anti-Integrin alpha 1 antibody, PA1045-1, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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Anti-Integrin alpha 1 antibody, PA1045-1, IHC(P)
IHC(P): Rat Intestine Tissue

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Anti-Integrin alpha 1 antibody, PA1045-1,Western blotting
All lanes: Anti ITGA1 (PA1045-1) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 131KD
Observed bind size: 131KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P56199
Gene Name ITGA1
Protein Name Integrin alpha-1
Alternative Names Integrin alpha-1;CD49 antigen-like family member A;Laminin and collagen receptor;VLA-1;CD49a;ITGA1;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 130848 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth. .
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background Integrin alpha 1(ITGA1) chain associates with the beta 1(ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

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In stock
Order Product
PA1045-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

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Publications

A Small Physiological Electric Field Mediated Responses of Extravillous Trophoblasts Derived from HTR8/SVneo Cells: Involvement of Activation of Focal Adhesion Kinase Signaling

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Why is my protein concentration value higher than expected when measuring absorbance at 280 nm? Keyword: absorption spectroscopy
    A: The antibody contains BSA, so the value tested was much higher than expected concentration. Other proteins the antibody contains make it difficult to test the concentration of target protein. Each vial of PA1045 contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. The antibody is affinity purified, and the antibody contains a small quantity of other rabbit IgG because purification rate cannot reach 100%.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: One other very common name is integrin beta-1 antibody
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